Rabbit anti-Human LOX1 Polyclonal Antibody | anti-LOX1 antibody

LOX1 (LOX-1, hLOX-1, C-type Lectin Domain Family 8 Member A, CLEC8A, Lectin-like Oxidized LDL Receptor 1, Lectin-like oxLDL Receptor 1, Lectin-type Oxidized LDL Receptor 1, LOXIN, Oxidized Low-density Lipoprotein Receptor 1, Ox-LDL Receptor 1, OLR1, SCARE

Cat. Num. AAA6485417

• Gene Names: OLR1; LOX1; LOXIN; SLOX1; CLEC8A; SCARE1
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: LOX1; Polyclonal Antibody; LOX1 (LOX-1; hLOX-1; C-type Lectin Domain Family 8 Member A; CLEC8A; Lectin-like Oxidized LDL Receptor 1; Lectin-like oxLDL Receptor 1; Lectin-type Oxidized LDL Receptor 1; LOXIN; Oxidized Low-density Lipoprotein Receptor 1; Ox-LDL Receptor 1; OLR1; SCARE; Oxidized Low Density Lipoprotein (Lectin-like) Receptor 1; anti-LOX1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human OLR1.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-LOX1 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-1:500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the center region of human OLR1 (KLH).
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of OLR1 Antibody (Center) in HL-60 cell line lysates (35ug/lane).OLR1 (arrow) was detected using the purified Pab.)

Western Blot (WB)

(Western blot analysis of OLR1 (arrow) using rabbit polyclonal OLR1 Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the OLR1 gene (Lane 2).)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human brain tissue reacted with OLR1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Product Categories/Family for anti-LOX1 antibody

Antibodies; Receptors

References

1. Predazzi, I.M., et.al., Ann. Hum. Biol. 37(2), 136-148 (2010).

NCBI and Uniprot Product Information

• NCBI GI #: 4505501
• NCBI GeneID: 4973
• NCBI Accession #: NP_002534.1
• NCBI GenBank Nucleotide #: NM_002543.3
• UniProt Accession #: P78380; Q2PP00; Q7Z484; A8K7V9; B4DI48; G3V1I4
• Molecular Weight: 30,959 Da
• NCBI Official Full Name: oxidized low-density lipoprotein receptor 1 isoform 1
• NCBI Official Synonym Full Names: oxidized low density lipoprotein (lectin-like) receptor 1
• NCBI Official Symbol: OLR1
• NCBI Official Synonym Symbols: LOX1; LOXIN; SLOX1; CLEC8A; SCARE1
• NCBI Protein Information: oxidized low-density lipoprotein receptor 1; hLOX-1; ox LDL receptor 1; lectin-type oxidized LDL receptor 1; scavenger receptor class E, member 1; C-type lectin domain family 8 member A; oxidized low-density lipoprotein receptor 1, soluble form
• UniProt Protein Name: Oxidized low-density lipoprotein receptor 1
• UniProt Gene Name: OLR1
• UniProt Synonym Gene Names: CLEC8A; LOX1; Ox-LDL receptor 1; LOX-1; Lectin-like oxLDL receptor 1; hLOX-1
• UniProt Entry Name: OLR1_HUMAN

NCBI Description

This gene encodes a low density lipoprotein receptor that belongs to the C-type lectin superfamily. This gene is regulated through the cyclic AMP signaling pathway. The encoded protein binds, internalizes and degrades oxidized low-density lipoprotein. This protein may be involved in the regulation of Fas-induced apoptosis. This protein may play a role as a scavenger receptor. Mutations of this gene have been associated with atherosclerosis, risk of myocardial infarction, and may modify the risk of Alzheimer's disease. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Feb 2010]

Uniprot Description

OLR1: Receptor that mediates the recognition, internalization and degradation of oxidatively modified low density lipoprotein (oxLDL) by vascular endothelial cells. OxLDL is a marker of atherosclerosis that induces vascular endothelial cell activation and dysfunction, resulting in pro-inflammatory responses, pro- oxidative conditions and apoptosis. Its association with oxLDL induces the activation of NF-kappa-B through an increased production of intracellular reactive oxygen and a variety of pro- atherogenic cellular responses including a reduction of nitric oxide (NO) release, monocyte adhesion and apoptosis. In addition to binding oxLDL, it acts as a receptor for the HSP70 protein involved in antigen cross-presentation to naive T-cells in dendritic cells, thereby participating in cell-mediated antigen cross-presentation. Also involved in inflammatory process, by acting as a leukocyte-adhesion molecule at the vascular interface in endotoxin-induced inflammation. Also acts as a receptor for advanced glycation end (AGE) products, activated platelets, monocytes, apoptotic cells and both Gram-negative and Gram- positive bacteria. Independent association genetic studies have implicated OLR1 gene variants in myocardial infarction susceptibility. OLR1 may be involved in Alzheimer disease (AD). Involvement in AD is however unclear: according to some authors (PubMed:12354387, PubMed:12810610 and PubMed:15976314), variations in OLR1 modify the risk of AD, while according to other (PubMed:15000751 and PubMed:15060104) they do not. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, misc.; Membrane protein, integral

Chromosomal Location of Human Ortholog: 12p13.2-p12.3

Cellular Component: nucleoplasm; intracellular membrane-bound organelle; membrane; integral to plasma membrane; plasma membrane; receptor complex; lipid raft

Molecular Function: low-density lipoprotein receptor activity; protein binding; carbohydrate binding

Biological Process: cell death; receptor-mediated endocytosis; leukocyte adhesion; response to hydrogen peroxide; blood circulation; lipoprotein metabolic process; proteolysis; blood coagulation; inflammatory response; leukocyte migration

Disease: Myocardial Infarction, Susceptibility To