Rabbit LOX Polyclonal Antibody | anti-LOX antibody

LOX Polyclonal Antibody

Cat. Num. AAA9128445

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity Purification
• Synonyms: LOX; Polyclonal Antibody; LOX Polyclonal Antibody; anti-LOX antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity Purification
• Sequence Length: 417

• Applicable Applications for anti-LOX antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB 1:500 - 1:2000; IHC 1:50 - 1:200
• Route: Synthetic Peptide
• Immunogen: A synthetic peptide of human LOX
• Preparation and Storage: Store at -20 degree C. Avoid freeze / thaw cycles.; Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using LOX antibody at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 90s.)

Related Product Information for anti-LOX antibody

The protein encoded by this gene is an extracellular copper enzyme that initiates the crosslinking of collagens and elastin. The enzyme catalyzes oxidative deamination of the epsilon-amino group in certain lysine and hydroxylysine residues of collagens and lysine residues of elastin. In addition to crosslinking extracellular matrix proteins, the encoded protein may have a role in tumor suppression. Defects in this gene are a cause of autosomal recessive cutis laxa type I (CL type I). Two transcript variants encoding different isoforms have been found for this gene.

NCBI and Uniprot Product Information

• NCBI GI #: 20149540
• NCBI GeneID: 4015
• NCBI Accession #: NP_002308.2
• NCBI GenBank Nucleotide #: NM_002317.5
• UniProt Accession #: P28300; Q5FWF0; B2R5Q3
• Molecular Weight: 47kDa
• NCBI Official Full Name: protein-lysine 6-oxidase isoform 1 preproprotein
• NCBI Official Synonym Full Names: lysyl oxidase
• NCBI Official Symbol: LOX
• NCBI Protein Information: protein-lysine 6-oxidase
• UniProt Protein Name: Protein-lysine 6-oxidase
• Protein Family: Linoleate 9/13-lipoxygenase
• UniProt Gene Name: LOX
• UniProt Entry Name: LYOX_HUMAN

NCBI Description

The protein encoded by this gene is an extracellular copper enzyme that initiates the crosslinking of collagens and elastin. The enzyme catalyzes oxidative deamination of the epsilon-amino group in certain lysine and hydroxylysine residues of collagens and lysine residues of elastin. In addition to crosslinking extracellular matrix proteins, the encoded protein may have a role in tumor suppression. Defects in this gene are a cause of autosomal recessive cutis laxa type I (CL type I). Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2010]

Uniprot Description

LOX: Responsible for the post-translational oxidative deamination of peptidyl lysine residues in precursors to fibrous collagen and elastin. In addition to cross-linking of extracellular matrix proteins, may have a direct role in tumor suppression. Belongs to the lysyl oxidase family.

Protein type: Secreted; Oxidoreductase; EC 1.4.3.13; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 5q23.2

Cellular Component: proteinaceous extracellular matrix; extracellular space; collagen; extracellular region; nucleus

Molecular Function: protein binding; copper ion binding; protein-lysine 6-oxidase activity

Biological Process: response to drug; blood vessel development; elastic fiber assembly; extracellular matrix organization and biogenesis; collagen fibril organization; wound healing; response to steroid hormone stimulus; protein modification process; lung development

Disease: Cutis Laxa, Autosomal Recessive, Type Ia