Rabbit anti-Human LOC552889 Polyclonal Antibody | anti-LOC552889 antibody

LOC552889, ID (ATXN7L3B, Putative ataxin-7-like protein 3B) (Azide free) (HRP)

Cat. Num. AAA6316684

• Gene Names: ATXN7L3B; lnc-SCA7
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: LOC552889; Polyclonal Antibody; ID (ATXN7L3B; Putative ataxin-7-like protein 3B) (Azide free) (HRP); ATXN7L3B; Putative ataxin-7-like protein 3B; anti-LOC552889 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-LOC552889 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: LOC552889 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 39-70 amino acids from the Central region of human LOC552889.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(LOC552889 Antibody (Center) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the LOC552889 antibody detected the LOC552889 protein (arrow).)

Flow Cytometry (FC/FACS)

(LOC552889 Antibody (Center) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-LOC552889 antibody

Antibodies; Ataxin

NCBI and Uniprot Product Information

• NCBI GI #: 210147443
• NCBI GeneID: 552889
• NCBI Accession #: NP_001129734.1
• NCBI GenBank Nucleotide #: NM_001136262.1
• UniProt Accession #: Q96GX2
• Molecular Weight: 10,771 Da
• NCBI Official Full Name: ataxin-7-like protein 3B
• NCBI Official Synonym Full Names: ataxin 7 like 3B
• NCBI Official Symbol: ATXN7L3B
• NCBI Official Synonym Symbols: lnc-SCA7
• NCBI Protein Information: ataxin-7-like protein 3B
• UniProt Protein Name: Ataxin-7-like protein 3B
• UniProt Gene Name: ATXN7L3B

Uniprot Description

By binding to ENY2, interferes with the nuclear functions of the deubiquitinase (DUB) module of the SAGA complex which consists of ENY2, ATXN7, ATXN7L3 and the histone deubiquitinating component USP22. Affects USP22 DUB activity toward histones indirectly by changing the subcellular distribution of ENY2 and altering ENY2 availability for ATXN7L3 interaction. Regulates H2B monoubiquitination (H2Bub1) levels through cytoplasmic sequestration of ENY2 resulting in loss of nuclear ENY2-ATXN7L3 association which destabilizes ATXN7L3. Affects protein expression levels of ENY2 and ATXN7L3.