Rabbit LMX1A Polyclonal Antibody | anti-LMX1A antibody

LMX1A Antibody

Cat. Num. AAA153489

• Gene Names: LMX1A; LMX1; LMX1.1
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: LMX1A Antibody is affinity chromatography purified via peptide column.
• Synonyms: LMX1A; Polyclonal Antibody; LMX1A Antibody; LMX1; LMX1.1; LIM homeobox transcription factor 1-alpha; LIM homeobox transcription factor 1; alpha; anti-LMX1A antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: At least two isoforms are known to exist. This antibody is predicted to not cross-react with LMX1B.
• Purity/Purification: LMX1A Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 382

• Applicable Applications for anti-LMX1A antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: LMX1A antibody can be used for detection of LMX1A by Western blot at 1 - 2 mug/mL. Antibody can also be used for Immunohistochemistry starting at 2.5 mug/mL. For immunofluorescence start at 20 mug/mL.
• Conjugate: Unconjugated
• Immunogen: Rabbit polyclonal LMX1A antibody was raised against a 16 amino acid peptide near the carboxy terminus of human LMX1A.
• Buffer: LMX1A Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: LMX1A antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of LMX1A in rat brain tissue lysate with LMX1A antibody at (A) 1 and (B) 2 μg/mL.)

Immunohistochemistry (IHC)

(Immunohistochemistry of LMX1A in human brain tissue with LMX1A antibody at 2.5 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of LMX1A in human brain tissue with LMX1A antibody at 20 μg/mL.)

Immunohistochemistry (IHC)

(Immunohistochemistry of LMX1A in mouse brain tissue with LMX1A Antibody at 5 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of LMX1A in mouse brain tissue with LMX1A Antibody at 20 μg/mL.Green: LMX1A Antibody (7087)Blue: DAPI staining)

Related Product Information for anti-LMX1A antibody

LMX1A Antibody: The LIM homeobox transcription factor 1 (LMX1A) belongs to the LIM-homeodomain family. Members of this family are known to be important for pattern formation during development. LMX1A binds an A/T-rich sequence and functions in the nucleus as a transcriptional activator to the insulin gene promoter. In the developing embryo, LMX1A is expressed along the neuraxis and leads to the development of the roof plate of the vertebrae. While LMX1A is crucial for the differentiation of dopamine neurons, the related LMX1B appears to be important for both the development and the survival of these cells. It has been suggested that LMX1A might be a useful biomarker or risk factor in tumor aggressiveness.

NCBI and Uniprot Product Information

• NCBI GI #: 28893581
• NCBI GeneID: 4009
• NCBI Accession #: NP_796372
• NCBI GenBank Nucleotide #: NM_177398.3
• UniProt Accession #: Q8TE12; Q0VDB5; Q5VWG4; Q8TE11; B3KXP6
• Molecular Weight: 42 kDa
• NCBI Official Full Name: LIM homeobox transcription factor 1-alpha
• NCBI Official Synonym Full Names: LIM homeobox transcription factor 1, alpha
• NCBI Official Symbol: LMX1A
• NCBI Official Synonym Symbols: LMX1; LMX1.1
• NCBI Protein Information: LIM homeobox transcription factor 1-alpha; LIM/homeobox protein 1.1
• UniProt Protein Name: LIM homeobox transcription factor 1-alpha
• Protein Family: LIM homeobox transcription factor
• UniProt Gene Name: LMX1A
• UniProt Synonym Gene Names: LMX-1.1
• UniProt Entry Name: LMX1A_HUMAN

NCBI Description

This gene encodes a homeodomain and LIM-domain containing protein. The encoded protein is a transcription factor that acts as a positive regulator of insulin gene transcription. This gene also plays a role in the development of dopamine producing neurons during embryogenesis. Mutations in this gene are associated with an increased risk of developing Parkinson's disease. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Feb 2012]

Uniprot Description

LMX1A: Acts as a transcriptional activator by binding to an A/T-rich sequence, the FLAT element, in the insulin gene promoter. Required for development of the roof plate and, in turn, for specification of dorsal cell fates in the CNS and developing vertebrae. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Cell development/differentiation; DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 1q24.1

Cellular Component: nucleus

Molecular Function: zinc ion binding; sequence-specific DNA binding

Biological Process: axon guidance; negative regulation of neuron differentiation; central nervous system neuron differentiation; dentate gyrus development; transcription, DNA-dependent; regulation of transcription, DNA-dependent; midbrain development; cerebellum development; regulation of cell growth