Rabbit anti-Human, Mouse Lmx1a Polyclonal Antibody | anti-Lmx1a antibody

Lmx1a, ID (LIM Homeobox Transcription Factor 1 alpha,LIM/Homeobox Protein LMX1A,LIM/Homeobox Protein 1.1,LMX-1.1, Lmx1a) (Azide free) (HRP)

Cat. Num. AAA6485129

• Gene Names: Lmx1a; dr; sst; Lmx1.1; dreher
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: Lmx1a; Polyclonal Antibody; ID (LIM Homeobox Transcription Factor 1 alpha; LIM/Homeobox Protein LMX1A; LIM/Homeobox Protein 1.1; LMX-1.1; Lmx1a) (Azide free) (HRP); anti-Lmx1a antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human LMX1a. Species Crossreactivity: mouse
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-Lmx1a antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 130-159 in the central region of human Lmx1a.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in HL60 cell line lysates (35ug/lane) usingMBS628381. Lmx1a (arrow) was detected usingMBS628381.)

Related Product Information for anti-Lmx1a antibody

Insulin is produced exclusively by the beta cells in the islets of Langerhans in the pancreas. The level and beta-cell specificity of insulin gene expression are regulated by a set of nuclear genes that bind to specific sequences within the promoter of the insulin gene (INS; MIM 176730) and interact with RNA polymerase to activate or repress transcription. LMX1 is a homeodomain protein that binds an A/T-rich sequence in the insulin promoter and stimulates transcription of insulin.

Product Categories/Family for anti-Lmx1a antibody

Antibodies; Transcription Factors; Homeobox (HOX)

References

1. Mangale, V.S., Science 319(5861), 304-309 (2008). 2. Robertson, E.J., Development 134(24), 4335-4345 (2007).

NCBI and Uniprot Product Information

• NCBI GI #: 17298670
• NCBI GeneID: 110648
• NCBI Accession #: NP_387501
• NCBI GenBank Nucleotide #: NM_033652
• UniProt Accession #: Q8TE12; Q9JKU8
• Molecular Weight: 43kDa
• NCBI Official Full Name: LIM homeobox transcription factor 1-alpha
• NCBI Official Synonym Full Names: LIM homeobox transcription factor 1 alpha
• NCBI Official Symbol: Lmx1a
• NCBI Official Synonym Symbols: dr; sst; Lmx1.1; dreher
• NCBI Protein Information: LIM homeobox transcription factor 1-alpha
• UniProt Protein Name: LIM homeobox transcription factor 1-alpha
• Protein Family: LIM homeobox transcription factor
• UniProt Gene Name: LMX1A
• UniProt Synonym Gene Names: LMX-1.1
• UniProt Entry Name: LMX1A_HUMAN

Uniprot Description

LMX1A: Acts as a transcriptional activator by binding to an A/T-rich sequence, the FLAT element, in the insulin gene promoter. Required for development of the roof plate and, in turn, for specification of dorsal cell fates in the CNS and developing vertebrae. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Cell development/differentiation; DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 1q24.1

Cellular Component: nucleus

Molecular Function: zinc ion binding; sequence-specific DNA binding

Biological Process: axon guidance; negative regulation of neuron differentiation; central nervous system neuron differentiation; dentate gyrus development; transcription, DNA-dependent; regulation of transcription, DNA-dependent; midbrain development; cerebellum development; regulation of cell growth