Western Blot (WB)
(Staining (1ug/ml) of Human Lung lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)
Goat LMO7/FBXO20 Polyclonal Antibody | anti-LMO7 antibody
Goat anti-LMO7/FBXO20 Antibody
Western Blot: Approx 70-75kDa band and approx 30kDa band observed in Human Lung lysates (predicted MWs of 92kDa according to AAD33924.1 - however our result agrees with Putilina et al as below). A minor band of unknown identity was also consistently observed at approx 50kDa. Recommended for use at 0.5-2ug/ml.
Western Blot (WB)
(Staining (1ug/ml) of Human Lung lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)
Western Blot (WB)
(Staining (1ug/ml) of Human Lung lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a protein containing a calponin homology (CH) domain, a PDZ domain, and a LIM domain, and may be involved in protein-protein interactions. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene, however, the full-length nature of some variants is not known. [provided by RefSeq, Jan 2009]
Uniprot Description
LMO7: contains a calponin homology (CH) domain, a PDZ domain, and a LIM domain; an F-box (FBX) domain is present in alternative splice variants. This protein is known to be upregulated in seven types of cancer. Translocates from the cell surface to the cytoplasm to the nucleus, regulates transcription of many genes, and may transmit mechanical signals from focal adhesions. Members of the LIM protein family carry the LIM domain, a unique cysteine-rich zinc-binding domain. Members of the FBX protein family are involved in protein-protein interactions. Four alternatively spliced isoforms have been described.
Protein type: Adaptor/scaffold
Chromosomal Location of Human Ortholog: 13q22.2
Cellular Component: cytoplasm; focal adhesion; nucleus
Research Articles on LMO7
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Product Notes
The LMO7 lmo7 (Catalog #AAA421148) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-LMO7/FBXO20 Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog and may cross-react with other species as described in the data sheet. AAA Biotech's LMO7/FBXO20 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 32000. Western Blot: Approx 70-75kDa band and approx 30kDa band observed in Human Lung lysates (predicted MWs of 92kDa according to AAD33924.1 - however our result agrees with Putilina et al as below). A minor band of unknown identity was also consistently observed at approx 50kDa. Recommended for use at 0.5-2ug/ml. Researchers should empirically determine the suitability of the LMO7 lmo7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: ESTCVSASLP RS. It is sometimes possible for the material contained within the vial of "LMO7/FBXO20, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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