Sheep anti-Human, Mouse LMO4 Polyclonal Antibody | anti-LMO4 antibody

LMO4 (Lim Region Containing Transcription Factor)

Cat. Num. AAA616983

• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by human LMO4 affinity chromatography.
• Synonyms: LMO4; Polyclonal Antibody; LMO4 (Lim Region Containing Transcription Factor); Anti -LMO4 (Lim Region Containing Transcription Factor); anti-LMO4 antibody

• Host: Sheep
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Selected for its ability to recognize human and mouse LMO4 in direct ELISAs and Western Blots.
• Purity/Purification: Affinity Purified; Purified by human LMO4 affinity chromatography.
• Form/Format: Supplied as a lyophilized powder from a 0.2um filtered solution in PBS with 5% trehalose. Reconstitute with sterile PBS, 40-50% glycerol. If 0.5ml is used, the concentration will be 0.2mg/ml.

• Applicable Applications for anti-LMO4 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Western Blot: This antibody can be used at 0.1-0.2ug/ml with the appropriate secondary reagents to detect human and mouse LMO4. The detection limit for rhLMO4 and rmLMO4 is approximately 10 ng/lane and 2 ng/lane under non-reducing and reducing conditions, respectively., Direct ELISA: This antibody can be used at 0.5-1.0ug/ml with the appropriate secondary reagents to detect human and mouse LMO4. The detection limit for rhLMO4 and rmLMO4 is approximately 1.5 ng/well., Optimal dilutions should be determined by each laboratory for each application.
• Immunogen: E. coli-derived recombinant human LMO4
• Preparation and Storage: Lyophilized powder may be stored at 4 degree C for short-term only. Reconstitute to nominal volume by adding sterile PBS with 40-50% glycerol and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-LMO4 antibody

LMO4 is a 18-20kD member of the LIM-only (LMO) subfamily of LIM domain-containing transcription factors. The 165 amino acid human LMO4 comprises a trans-activating Pro-rich N-terminus, two LIM domains, and a LIM-binding C-terminus. The LIM domain is a conserved cystein-rich structure containing two tandemly repeated zinc fingers involved in protein-protein interaction. LIMs are found in a variety of proteins known to have critical functions in cell-fate specification and differentiation. LMO4 is both nuclear and cytoplasmic. LMO4 is overexpressed in most primary breast carcinomas. Human and mouse LMO4 protein sequences are 100% identical.

Product Categories/Family for anti-LMO4 antibody

Antibodies; Abs to Transcription Factors; Zinc (Ring) Finger

NCBI and Uniprot Product Information

• NCBI GI #: 5803072
• NCBI GeneID: 8543
• NCBI Accession #: NP_006760.1
• NCBI GenBank Nucleotide #: NM_006769.3
• UniProt Accession #: P61968; O00158; O88894; D3DT23
• Molecular Weight: 17,994 Da
• NCBI Official Full Name: LIM domain transcription factor LMO4
• NCBI Official Synonym Full Names: LIM domain only 4
• NCBI Official Symbol: LMO4
• NCBI Protein Information: LIM domain transcription factor LMO4; LMO-4; OTTHUMP00000011905; OTTHUMP00000011906; breast tumor autoantigen; LIM domain only protein 4
• UniProt Protein Name: LIM domain transcription factor LMO4
• Protein Family: LIM domain transcription factor
• UniProt Gene Name: LMO4
• UniProt Entry Name: LMO4_HUMAN

NCBI Description

This gene encodes a cysteine-rich protein that contains two LIM domains but lacks a DNA-binding homeodomain. The encoded protein may play a role as a transcriptional regulator or as an oncogene. [provided by RefSeq]

Uniprot Description

LMO4: Probable transcriptional factor.

Protein type: Transcription factor

Chromosomal Location of Human Ortholog: 1p22.3

Cellular Component: transcription factor complex

Molecular Function: zinc ion binding; transcription factor binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; thymus development; ventral spinal cord interneuron differentiation; regulation of cell fate specification; regulation of cell activation; neural tube closure; spinal cord association neuron differentiation; positive regulation of transcription from RNA polymerase II promoter; spinal cord motor neuron differentiation; negative regulation of protein complex assembly