Immunofluorescence (IF)
(Fluorescent image of A549 cell stained with LMO4 Antibody (Center).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with LMO4 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃).LMO4 immunoreactivity is localized to Nucleus significantly.)
Rabbit anti-Human, Mouse LMO4 Polyclonal Antibody | anti-LMO4 antibody
LMO4, ID (LMO4, LIM domain transcription factor LMO4, Breast tumor autoantigen, LIM domain only protein 4)
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Flow Cytometry: 1:10-50
Immunofluorescence (IF)
(Fluorescent image of A549 cell stained with LMO4 Antibody (Center).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with LMO4 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃).LMO4 immunoreactivity is localized to Nucleus significantly.)
Immunofluorescence (IF)
(Fluorescent image of A549 cell stained with LMO4 Antibody (Center).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with LMO4 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃).LMO4 immunoreactivity is localized to Nucleus significantly.)
Western Blot (WB)
(LMO4 Antibody (Center) western blot analysis in mouse spleen tissue lysates (35ug/lane).This demonstrates the LMO4 antibody detected the LMO4 protein (arrow).)
Western Blot (WB)
(LMO4 Antibody (Center) western blot analysis in mouse spleen tissue lysates (35ug/lane).This demonstrates the LMO4 antibody detected the LMO4 protein (arrow).)
Flow Cytometry (FC/FACS)
(LMO4 Antibody (Center) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(LMO4 Antibody (Center) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a cysteine-rich protein that contains two LIM domains but lacks a DNA-binding homeodomain. The encoded protein may play a role as a transcriptional regulator or as an oncogene. [provided by RefSeq, Aug 2008]
Uniprot Description
LMO4: Probable transcriptional factor.
Protein type: Transcription factor
Chromosomal Location of Human Ortholog: 1p22.3
Cellular Component: transcription factor complex
Molecular Function: zinc ion binding; transcription factor binding; transcription factor activity
Biological Process: transcription from RNA polymerase II promoter; thymus development; ventral spinal cord interneuron differentiation; neural tube closure; regulation of cell activation; regulation of cell fate specification; spinal cord association neuron differentiation; positive regulation of transcription from RNA polymerase II promoter; spinal cord motor neuron differentiation; negative regulation of protein complex assembly
Research Articles on LMO4
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Product Notes
The LMO4 lmo4 (Catalog #AAA6006376) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The LMO4, ID (LMO4, LIM domain transcription factor LMO4, Breast tumor autoantigen, LIM domain only protein 4) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's LMO4 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Flow Cytometry (FC/FACS). Suitable for use in Western Blot, Flow Cytometry, ELISA Dilution: ELISA: 1:1,000 Western Blot: 1:100-500 Flow Cytometry: 1:10-50. Researchers should empirically determine the suitability of the LMO4 lmo4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LMO4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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