Rabbit Livin Polyclonal Antibody
Livin Polyclonal Antibody
Applications
Immunoblot, Western Blot
Synonyms
Livin; Polyclonal Antibody; Livin Polyclonal Antibody; anti-Livin antibody
Host
Rabbit
Clonality
Polyclonal
Specificity
Livin [This rabbit antibody recognizes aa 264-80 (short form) and aa 271-298 (long form) of human Livin.]
This antibody recognizes human Livin, a novel member of the IAP protein family. Livin contains a single baculoviral IAP repeat domain and a RING finger domain and has two isoforms, Livin- alpha and Livin- beta . Transfection of Livin in cells results in protection from apoptosis induced by FADD, Bax, RIP, RIP3, and DR6. Livin interacts directly with caspase-3, -7, and -9 and inhibits the activation of caspase-9 induced by Apaf-1, cytochrome c, and dATP.
This antibody recognizes human Livin, a novel member of the IAP protein family. Livin contains a single baculoviral IAP repeat domain and a RING finger domain and has two isoforms, Livin- alpha and Livin- beta . Transfection of Livin in cells results in protection from apoptosis induced by FADD, Bax, RIP, RIP3, and DR6. Livin interacts directly with caspase-3, -7, and -9 and inhibits the activation of caspase-9 induced by Apaf-1, cytochrome c, and dATP.
Form/Format
100 ug IgG in PBS, pH 7.4, purified by immunoaffinity chromatography.
Applicable Applications for anti-Livin antibody
Immunoblot
Application Notes
Immunoblotting: use at 2-4 ug/ml. In immunoblots, a band of 33 kD is detected. A weak band at 30 kD is detected in Raji cell lysate and probably represents the short form of human Livin.
Positive control: Raji cell lysate.
Positive control: Raji cell lysate.
Antigen
Peptide corresponding to aa 264-280 of the short form and aa 281-298 of the long form of human Livin.
Stabilizer
None
Preservatives
0.02% sodium azide
Dilutions Instructions
Dilute in PBS or medium which is identical to that used in the assay system.
Preparation and Storage
This antibody is stable for at least one (1) year at -20 degree C. Avoid multiple freeze-thaw cycles.
Product Categories/Family for anti-Livin antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
LIVIN
NCBI Description
This gene encodes a member of the inhibitor of apoptosis protein (IAP) family, and contains a single copy of a baculovirus IAP repeat (BIR) as well as a RING-type zinc finger domain. The BIR domain is essential for inhibitory activity and interacts with caspases, while the RING finger domain sometimes enhances antiapoptotic activity but does not inhibit apoptosis alone. Elevated levels of the encoded protein may be associated with cancer progression and play a role in chemotherapy sensitivity. Alternative splicing results in multiple transcript variants [provided by RefSeq, Jul 2013]
Similar Products
Product Notes
The Livin (Catalog #AAA194296) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Livin can be used in a range of immunoassay formats including, but not limited to, Immunoblot. Immunoblotting: use at 2-4 ug/ml. In immunoblots, a band of 33 kD is detected. A weak band at 30 kD is detected in Raji cell lysate and probably represents the short form of human Livin. Positive control: Raji cell lysate. Researchers should empirically determine the suitability of the Livin for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Livin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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