Rabbit Cold Inducible RNA Binding Protein Polyclonal Antibody | anti-CIRBP antibody

Polyclonal Antibody to Cold Inducible RNA Binding Protein (CIRBP)

Cat. Num. AAA2026125

• Gene Names: CIRBP; CIRP
• Reactivity: Homo sapiens (Human)
• Applications: Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
• Purity: Antigen-specific affinity chromatography followed by Protein A affinity chromatography
• Synonyms: Cold Inducible RNA Binding Protein; Polyclonal Antibody; Polyclonal Antibody to Cold Inducible RNA Binding Protein (CIRBP); anti-CIRBP antibody

• Host: Rabbit
• Reactivity: Homo sapiens (Human)
• Clonality: Polyclonal
• Purity/Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography
• Form/Format: Supplied as solution form in 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
• Concentration: 0.43mg/ml (varies by lot)

• Applicable Applications for anti-CIRBP antibody: Western Blot (WB); Immunohistochemistry (IHC); Immunocytochemistry (ICC); Immunoprecipitation (IP).
• Application Notes: Western blotting: 0.5-2ug/mL; Immunohistochemistry: 5-20ug/mL; Immunocytochemistry: 5-20ug/mL; Optimal working dilutions must be determined by end user.
• Source: Polyclonal antibody preparation
• Traits: Liquid
• Cross Reactivity: Mouse, Rat, Pig
• Immunogen: Recombinant CIRBP (Met1~Glu172) expressed in E.coli (MBS2030280).
• Preparation and Storage: Storage:; Avoid repeated freeze/thaw cycles.; Store at 4 degree C for frequent use.; Aliquot and store at -20 degree C for 24 months.; ; Stability Test:; thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Western Blot (WB)

(Western Blot: Sample: Recombinant CIRBP, Human.)

Western Blot (WB)

(Western Blot:Sample: Lane 1 Mouse Brain TissueLane 2: Porcine Brain Tissue.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P;Samples: Human Skin cancer Tissue;Primary Ab: 10ug/ml Rabbit Anti-Human CIRBP AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047))

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Human Stomach Tissue.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Human Kidney Tissue)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Human Prostate Gland Cancer Tissue)

NCBI and Uniprot Product Information

• NCBI GI #: 4502847
• NCBI GeneID: 1153
• NCBI GenBank Nucleotide #: NM_001280.2
• NCBI Official Full Name: cold-inducible RNA-binding protein isoform 1
• NCBI Official Synonym Full Names: cold inducible RNA binding protein
• NCBI Official Symbol: CIRBP
• NCBI Official Synonym Symbols: CIRP
• NCBI Protein Information: cold-inducible RNA-binding protein
• UniProt Protein Name: Cold-inducible RNA-binding protein
• Protein Family: Cold-inducible RNA-binding protein
• UniProt Gene Name: CIRBP
• UniProt Synonym Gene Names: A18HNRNP; CIRP

Uniprot Description

Cold-inducible mRNA binding protein that plays a protective role in the genotoxic stress response by stabilizing transcripts of genes involved in cell survival. Acts as a translational activator. Seems to play an essential role in cold-induced suppression of cell proliferation. Binds specifically to the 3'-untranslated regions (3'-UTRs) of stress-responsive transcripts RPA2 and TXN. Acts as a translational repressor (). Promotes assembly of stress granules (SGs), when overexpressed.