Rabbit DcR1 Polyclonal Antibody | anti-TNFRSF10C antibody

DcR1 Antibody

Cat. Num. AAA150747

• Gene Names: TNFRSF10C; LIT; DCR1; TRID; CD263; TRAILR3; TRAIL-R3; DCR1-TNFR
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunofluorescence
• Purity: DcR1 Antibody is affinity chromatography purified via peptide column.
• Synonyms: DcR1; Polyclonal Antibody; DcR1 Antibody; LIT; DCR1; TRID; CD263; TRAILR3; TRAIL-R3; DCR1-TNFR; UNQ321/PRO366; Tumor necrosis factor receptor superfamily member 10C; Decoy TRAIL receptor without death domain; tumor necrosis factor receptor superfamily; member 10c; decoy without an intracellular domain; anti-TNFRSF10C antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: DcR1 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Sequence Length: 1180

• Applicable Applications for anti-TNFRSF10C antibody: ELISA (EIA), Western Blot (WB), Immunofluorescence (IF)
• Application Notes: DcR1 antibody can be used for detection of DcR1 by Western blot at 1 mug/mL. An approximate 65 kDa band can be detected. Antibody can also be used for immunoflourescence starting at 10 mug/mL. For immunofluorescence start at 20 mug/mL.
• Conjugate: Unconjugated
• Immunogen: DcR1 antibody was raised against a peptide corresponding to amino acids in the extracellular domain of human DcR1 precursor.
• Buffer: DcR1 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: DcR1 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of DcR1 in HeLa cell (A), mouse (B) and rat (C) liver tissue lysates with DcR1 antibody at 1 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of DcR1 in rat liver tissue with DcR1 antibody at 10 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of DcR1 in Rat Liver cells with DcR1 antibody at 20 μg/mL.)

Related Product Information for anti-TNFRSF10C antibody

DcR1 Antibody: Apoptosis is induced by certain cytokines including TNF and Fas ligand in the TNF family through their death domain containing receptors. TRAIL/Apo2L is a new member of the TNF family and induces apoptosis of a variety of tumor cell lines. DR4 and DR5 are the recently identified functional receptors for TRAIL. Two decoy receptors for TRAIL have been identified and designated DcR1/TRID/TRAIL-R3/LIT and DcR2/TRAIL-R4/TRUNDD. DcR1 has extracellular TRAIL-binding domain but lacks intracellular signaling domain. It is a glycophospholipid-anchored cell surface protein. DcR1 transcripts are expressed in many normal human tissues but not in most cancer cell lines. Overexpression of DcR1 did not induce apoptosis, but attenuated TRAIL-induced apoptosis.

NCBI and Uniprot Product Information

• NCBI GI #: 2338421
• NCBI GeneID: 8794
• UniProt Accession #: O14798; O14755; Q08AS6; Q6FH98; Q6UXM5
• Molecular Weight: 65 kDa
• NCBI Official Full Name: Homo sapiens decoy receptor 1 (DcR1) mRNA, complete cds
• NCBI Official Synonym Full Names: tumor necrosis factor receptor superfamily, member 10c, decoy without an intracellular domain
• NCBI Official Symbol: TNFRSF10C
• NCBI Official Synonym Symbols: LIT; DCR1; TRID; CD263; TRAILR3; TRAIL-R3; DCR1-TNFR
• NCBI Protein Information: tumor necrosis factor receptor superfamily member 10C; decoy receptor 1; cytotoxic TRAIL receptor-3; lymphocyte inhibitor of TRAIL; decoy TRAIL receptor without death domain; antagonist decoy receptor for TRAIL/Apo-2L; TNF-related apoptosis-inducing ligand receptor 3
• UniProt Protein Name: Tumor necrosis factor receptor superfamily member 10C
• Protein Family: Tumor necrosis factor receptor superfamily
• UniProt Gene Name: TNFRSF10C
• UniProt Synonym Gene Names: DCR1; LIT; TRAILR3; TRID; DcR1; TRAIL receptor 3; TRAIL-R3
• UniProt Entry Name: TR10C_HUMAN

NCBI Description

The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor contains an extracellular TRAIL-binding domain and a transmembrane domain, but no cytoplasmic death domain. This receptor is not capable of inducing apoptosis, and is thought to function as an antagonistic receptor that protects cells from TRAIL-induced apoptosis. This gene was found to be a p53-regulated DNA damage-inducible gene. The expression of this gene was detected in many normal tissues but not in most cancer cell lines, which may explain the specific sensitivity of cancer cells to the apoptosis-inducing activity of TRAIL. [provided by RefSeq, Jul 2008]

Uniprot Description

TRAIL-R3: Receptor for the cytotoxic ligand TRAIL. Lacks a cytoplasmic death domain and hence is not capable of inducing apoptosis. May protect cells against TRAIL mediated apoptosis by competing with TRAIL-R1 and R2 for binding to the ligand. Higher expression in normal tissues than in tumor cell lines. Highly expressed in peripheral blood lymphocytes, spleen, skeletal muscle, placenta, lung and heart.

Protein type: Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: 8p22-p21

Cellular Component: integral to plasma membrane

Molecular Function: transmembrane receptor activity; TRAIL binding

Biological Process: apoptosis; signal transduction