Rabbit anti-Human, Mouse LIN7B Polyclonal Antibody | anti-LIN7B antibody

LIN7B, NT (LIN7B, MALS2, VELI2, Protein lin-7 homolog B, Mammalian lin-seven protein 2, Vertebrate lin-7 homolog 2) (Biotin)

Cat. Num. AAA6316286

• Gene Names: LIN7B; MALS2; VELI2; LIN-7B; MALS-2
• Reactivity: Human, Mouse
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: LIN7B; Polyclonal Antibody; NT (LIN7B; MALS2; VELI2; Protein lin-7 homolog B; Mammalian lin-seven protein 2; Vertebrate lin-7 homolog 2) (Biotin); Vertebrate lin-7 homolog 2; anti-LIN7B antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-LIN7B antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: LIN7B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 35-62 amino acids from the N-terminal region of human LIN7B.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(LIN7B Antibody (N-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the LIN7B antibody detected the LIN7B protein (arrow).)

Western Blot (WB)

(LIN7B Antibody (N-term) western blot analysis in mouse cerebellum tissue lysates (35ug/lane).This demonstrates the LIN7B antibody detected the LIN7B protein (arrow).)

Related Product Information for anti-LIN7B antibody

Plays a role in establishing and maintaining the asymmetric distribution of channels and receptors at the plasma membrane of polarized cells. Forms membrane-associated multiprotein complexes that may regulate delivery and recycling of proteins to the correct membrane domains. The tripartite complex composed of LIN7 (LIN7A, LIN7B or LIN7C), CASK and APBA1 may have the potential to couple synaptic vesicle exocytosis to cell adhesion in brain. Ensures the proper localization of GRIN2B (subunit 2B of the NMDA receptor) to neuronal postsynaptic density and may function in localizing synaptic vesicles at synapses where it is recruited by beta-catenin and cadherin. Required to localize Kir2 channels, GABA transporter (SLC6A12) and EGFR/ERBB1, ERBB2, ERBB3 and ERBB4 to the basolateral membrane of epithelial cells. May increase the amplitude of ACCN3 acid-evoked currents by stabilizing the channel at the cell surface (By similarity).

Product Categories/Family for anti-LIN7B antibody

Antibodies; Proteins

References

Zucker, B., et al. J. Neuropathol. Exp. Neurol. 69(9):880-895(2010) Lanktree, M., et al. Am. J. Med. Genet. B Neuropsychiatr. Genet. 147B (6), 945-951 (2008): Sudo, K., et al. Neurosci. Res. 56(4):347-355(2006) Li, Z., et al. J. Biol. Chem. 281(16):11066-11073(2006) Kawai, S., et al. J. Biol. Chem. 280(47):39200-39207(2005).

NCBI and Uniprot Product Information

• NCBI GI #: 11545920
• NCBI GeneID: 64130
• NCBI Accession #: NP_071448.1
• NCBI GenBank Nucleotide #: NM_022165.2
• UniProt Accession #: Q9HAP6
• Molecular Weight: 15,620 Da
• NCBI Official Full Name: protein lin-7 homolog B
• NCBI Official Synonym Full Names: lin-7 homolog B (C. elegans)
• NCBI Official Symbol: LIN7B
• NCBI Official Synonym Symbols: MALS2; VELI2; LIN-7B; MALS-2
• NCBI Protein Information: protein lin-7 homolog B; hLin7B; hVeli2; mammalian lin-seven protein 2; veli-2; vertebrate lin-7 homolog 2
• UniProt Protein Name: Protein lin-7 homolog B
• Protein Family: Protein
• UniProt Gene Name: LIN7B
• UniProt Synonym Gene Names: MALS2; VELI2; Lin-7B; hLin7B; MALS-2; Veli-2; hVeli2
• UniProt Entry Name: LIN7B_HUMAN

Uniprot Description

LIN7B: Plays a role in establishing and maintaining the asymmetric distribution of channels and receptors at the plasma membrane of polarized cells. Forms membrane-associated multiprotein complexes that may regulate delivery and recycling of proteins to the correct membrane domains. The tripartite complex composed of LIN7 (LIN7A, LIN7B or LIN7C), CASK and APBA1 may have the potential to couple synaptic vesicle exocytosis to cell adhesion in brain. Ensures the proper localization of GRIN2B (subunit 2B of the NMDA receptor) to neuronal postsynaptic density and may function in localizing synaptic vesicles at synapses where it is recruited by beta-catenin and cadherin. Required to localize Kir2 channels, GABA transporter (SLC6A12) and EGFR/ERBB1, ERBB2, ERBB3 and ERBB4 to the basolateral membrane of epithelial cells. May increase the amplitude of ASIC3 acid-evoked currents by stabilizing the channel at the cell surface. Belongs to the lin-7 family. 2 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 19q13.3

Cellular Component: postsynaptic membrane; neuron projection; tight junction; basolateral plasma membrane; postsynaptic density; synapse; intercellular junction

Molecular Function: protein domain specific binding

Biological Process: asymmetric protein localization; protein transport; exocytosis; neurotransmitter secretion; maintenance of epithelial cell polarity