Rabbit anti-Human lin28b Polyclonal Antibody | anti-lin28b antibody

lin28b Antibody

Cat. Num. AAA9613597

• Gene Names: LIN28B; CSDD2
• Reactivity: Human
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
• Synonyms: lin28b; Polyclonal Antibody; lin28b Antibody; CSDD 2; CSDD2; FLJ16517; Lin 28 homolog B (C. elegans); Lin 28.2; Lin-28B; LIN28B; LN28B_HUMAN; Protein lin-28 homolog B; anti-lin28b antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: lin28b Antibody detects endogenous levels of total lin28b.; Tissue Specificity: Expressed at high levels in the placenta and, at mucher lower, in testis and fetal liver. Isoform 1 is only detected in placenta and in moderately and poorly differentiated hepatocellular carcinoma cells (at protein level). Isoform 2 is detected in fetal liver, non-tumor liver tissues, as well as well-differentiated tumor tissues (at protein level). Tends to be up-regulated in triple-negative (ER-,PR-,HER2-) breast tumors, as well as in liver, ovarian, and thyroid carcinomas.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-lin28b antibody: Peptide ELISA (EIA)
• Application Notes: Peptide ELISA: 1:20,000-1:40,000
• Immunogen: A synthesized peptide derived from human lin28b.
• Conjugation: Unconjugated
• Fragment: Fab fragment
• Similarity: The tandem zinc fingers, also referred as zinc knuckle domain (ZKD), mediate specific binding to the GGAG/GGUG motif while the CSD shows only limited pyrimidine-rich sequence specificity. Both domains bind single-stranded nucleic acids.Belongs to the lin-28 family.
• Subcellular Location: Nucleus. Nucleus>Nucleolus. Cytoplasm.; Note: Predominantly nucleolar (PubMed:22118463). In Huh7 cells, predominantly cytoplasmic, with only a subset of cells exhibiting strong nuclear staining; however, the specificity of the polyclonal antibody used in these experiments has not been not documented (PubMed:16971064).
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Related Product Information for anti-lin28b antibody

Suppressor of microRNA (miRNA) biogenesis, including that of let-7 and possibly of miR107, miR-143 and miR-200c. Binds primary let-7 transcripts (pri-let-7), including pri-let-7g and pri-let-7a-1, and sequester them in the nucleolus, away from the microprocessor complex, hence preventing their processing into mature miRNA. Does not act on pri-miR21. The repression of let-7 expression is required for normal development and contributes to maintain the pluripotent state of embryonic stem cells by preventing let-7-mediated differentiation. When overexpressed, recruits ZCCHC11/TUT4 uridylyltransferase to pre-let-7 transcripts, leading to their terminal uridylation and degradation. This activity might not be relevant in vivo, as LIN28B-mediated inhibition of let-7 miRNA maturation appears to be ZCCHC11-independent. Interaction with target pre-miRNAs occurs via an 5'-GGAG-3' motif in the pre-miRNA terminal loop. Mediates MYC-induced let-7 repression (By similarity). When overexpressed, isoform 1 stimulates growth of the breast adenocarcinoma cell line MCF-7. Isoform 2 has no effect on cell growth.

NCBI and Uniprot Product Information

• NCBI GI #: 51972214
• NCBI GeneID: 389421
• NCBI Accession #: NP_001004317.1
• NCBI GenBank Nucleotide #: NM_001004317.3
• UniProt Accession #: Q6ZN17; Q5TCM4; A1L165; B2RPN6
• Molecular Weight: 19,511 Da
• NCBI Official Full Name: protein lin-28 homolog B
• NCBI Official Synonym Full Names: lin-28 homolog B (C. elegans)
• NCBI Official Symbol: LIN28B
• NCBI Official Synonym Symbols: CSDD2
• NCBI Protein Information: protein lin-28 homolog B; Lin-28.2; lin-28B
• UniProt Protein Name: Protein lin-28 homolog B
• Protein Family: Protein
• UniProt Gene Name: LIN28B
• UniProt Synonym Gene Names: CSDD2; Lin-28B
• UniProt Entry Name: LN28B_HUMAN

NCBI Description

The protein encoded by this gene belongs to the lin-28 family, which is characterized by the presence of a cold-shock domain and a pair of CCHC zinc finger domains. This gene is highly expressed in testis, fetal liver, placenta, and in primary human tumors and cancer cell lines. It is negatively regulated by microRNAs that target sites in the 3' UTR, and overexpression of this gene in primary tumors is linked to the repression of let-7 family of microRNAs and derepression of let-7 targets, which facilitates cellular transformation. [provided by RefSeq, Jun 2012]

Uniprot Description

LIN28B: Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre- let-7. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Specifically recognizes the 5'-GGAG-3' motif in the terminal loop of pre-let-7. Also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5'-GGAG-3' motif in the terminal loop, leading to their terminal uridylation and subsequent degradation. Mediates MYC-mediated let-7 repression. Isoform 1, when overexpressed, stimulates growth of the breast adenocarcinoma cell line MCF-7. Isoform 2 has no effect on cell growth. Interacts with ZCCHC11/TUT4. Might be negatively regulated by the microRNA let-7b. High expression in testis, fetal liver, placenta and in hepatocellular carcinoma (HCC). Isoform 1 is only detected in moderately and poorly differentiated HCC tissues and placenta. Isoform 2 is detected in fetal liver, non-tumor liver tissues, as well as well-differentiated tumor tissues. Belongs to the lin-28 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Unknown function

Chromosomal Location of Human Ortholog: 6q21

Cellular Component: nucleoplasm; mitochondrion; cytoplasm; nucleolus; nucleus

Molecular Function: protein binding; DNA binding; zinc ion binding; RNA binding

Biological Process: regulation of transcription, DNA-dependent; pre-microRNA processing; RNA 3'-end processing