Rabbit anti-Human, Mouse LIN28 Polyclonal Antibody | anti-LIN28 antibody

LIN28 (LIN28A, CSDD1, LIN28, ZCCHC1, Protein lin-28 homolog A, Zinc finger CCHC domain-containing protein 1) (Biotin)

Cat. Num. AAA6316198

• Gene Names: LIN28A; CSDD1; LIN28; LIN-28; ZCCHC1; lin-28A
• Reactivity: Human, Mouse
• Applications: Flow Cytometry, Functional Assay, ELISA, Western Blot
• Purity: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Synonyms: LIN28; Polyclonal Antibody; LIN28 (LIN28A; CSDD1; ZCCHC1; Protein lin-28 homolog A; Zinc finger CCHC domain-containing protein 1) (Biotin); LIN28A; Zinc finger CCHC domain-containing protein 1; anti-LIN28 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-LIN28 antibody: Flow Cytometry (FC/FACS), ELISA (EIA), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; ELISA: 1:1,000; WB: 1:50-100; Applications are based on unconjugated antibody.
• Immunogen: LIN28A antibody is generated from rabbits immunized with a His fusion protein from human LIN28A.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(LIN28 Antibody western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the LIN28 antibody detected the LIN28 protein (arrow).)

Western Blot (WB)

(LIN28 Antibody western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).This demonstrates the LIN28 antibody detected the LIN28 protein (arrow).)

Flow Cytometry (FC/FACS)

(LIN28 Antibody flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-LIN28 antibody

Acts as a 'translational enhancer', driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis. Its association with the translational machinery and target mRNAs results in an increased number of initiation events per molecule of mRNA and, indirectly, in stabilizing the mRNAs. Binds IGF2 mRNA, MYOD1 mRNA, ARBP/36B4 ribosomal protein mRNA and its own mRNA. Essential for skeletal muscle differentiation program through the translational up-regulation of IGF2 expression (By similarity). Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre-let-7. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Degradation of pre-let-7 in embryonic stem (ES) cells contributes to the maintenance of ES cells. In contrast, LIN28A down-regulation in neural stem cells by miR-125, allows the processing of pre-let-7. Specifically recognizes the 5'-GGAG-3' motif in the terminal loop of pre-let-7. Also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5'-GGAG-3' motif in the terminal loop, leading to their terminal uridylation and subsequent degradation.

Product Categories/Family for anti-LIN28 antibody

Antibodies; Transcription Factors; Zinc (Ring) Finger

References

Peng, S., et al. Oncogene 29(14):2153-2159(2010). Qiu, C., et al. Nucleic Acids Res. 38(4):1240-1248(2010). Iliopoulos, D., et al. Cell 139(4):693-706(2009). Heo, I., et al. Cell 138(4):696-708(2009). West, J.A., et al. Nature 460(7257):909-913(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 13375938
• NCBI GeneID: 79727
• NCBI Accession #: NP_078950.1
• NCBI GenBank Nucleotide #: NM_024674.4
• UniProt Accession #: Q9H9Z2
• Molecular Weight: 22,743 Da
• NCBI Official Full Name: protein lin-28 homolog A
• NCBI Official Synonym Full Names: lin-28 homolog A (C. elegans)
• NCBI Official Symbol: LIN28A
• NCBI Official Synonym Symbols: CSDD1; LIN28; LIN-28; ZCCHC1; lin-28A
• NCBI Protein Information: protein lin-28 homolog A; RNA-binding protein LIN-28; zinc finger CCHC domain-containing protein 1; zinc finger, CCHC domain containing 1
• UniProt Protein Name: Protein lin-28 homolog A
• UniProt Gene Name: LIN28A
• UniProt Synonym Gene Names: CSDD1; LIN28; ZCCHC1; Lin-28A
• UniProt Entry Name: LN28A_HUMAN

Uniprot Description

LIN28A: Acts as a 'translational enhancer', driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis. Its association with the translational machinery and target mRNAs results in an increased number of initiation events per molecule of mRNA and, indirectly, in stabilizing the mRNAs. Binds IGF2 mRNA, MYOD1 mRNA, ARBP/36B4 ribosomal protein mRNA and its own mRNA. Essential for skeletal muscle differentiation program through the translational up-regulation of IGF2 expression. Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let- 7), a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre-let-7. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Degradation of pre- let-7 in embryonic stem (ES) cells contributes to the maintenance of ES cells. In contrast, LIN28A down-regulation in neural stem cells by miR-125, allows the processing of pre-let-7. Specifically recognizes the 5'-GGAG-3' motif in the terminal loop of pre-let-7. Also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5'-GGAG-3' motif in the terminal loop, leading to their terminal uridylation and subsequent degradation. Monomer. During skeletal muscle differentiation, associated with translation initiation complexes in the polysomal compartment. Directly interacts with EIF3S2. Interaction with NCL is RNA-dependent. Interacts with ZCCHC11/TUT4. Can be negatively regulated by the interaction of microRNAs miR-125a and miR-125b with at least two miRNA responsive elements (miREs) in the 3'-UTR of this gene. These interactions may reduce both translation efficiency and mRNA abundance. Negatively regulated by retinoic acid. Expressed in embryonic stem cells (ES cells), placenta and testis. Belongs to the lin-28 family.

Protein type: Translation; RNA-binding; Nucleolus

Chromosomal Location of Human Ortholog: 1p36.11

Cellular Component: stress granule; cytoplasm; nucleolus; nucleus; cytosol

Molecular Function: mRNA binding; protein binding; miRNA binding; DNA binding; zinc ion binding; RNA binding; translation initiation factor binding

Biological Process: positive regulation of translation; regulation of transcription, DNA-dependent; pre-microRNA processing; somatic stem cell maintenance; stem cell maintenance; positive regulation of neuron differentiation; RNA 3'-end processing; germ cell development; negative regulation of glial cell differentiation