Western Blot (WB)
(All lanes : Anti-HDAC1 Antibody (K464) at 1:2000 dilutionLane 1: Hela whole cell lysatesLane 2: Jurkat whole cell lysatesLane 3: K562 whole cell lysatesLane 4: mouse thymus lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 55 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Rabbit HDAC1 Polyclonal Antibody | anti-HDAC1 antibody
HDAC1 Antibody (C-term)
Western Blot (WB)
(All lanes : Anti-HDAC1 Antibody (K464) at 1:2000 dilutionLane 1: Hela whole cell lysatesLane 2: Jurkat whole cell lysatesLane 3: K562 whole cell lysatesLane 4: mouse thymus lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 55 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(All lanes : Anti-HDAC1 Antibody (K464) at 1:2000 dilutionLane 1: Hela whole cell lysatesLane 2: Jurkat whole cell lysatesLane 3: K562 whole cell lysatesLane 4: mouse thymus lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 55 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(All lanes : Anti-HDAC1 Antibody (K464) at 1:2000 dilutionLane 1: Hela whole cell lysatesLane 2: Jurkat whole cell lysatesLane 3: K562 whole cell lysatesLane 4: mouse thymus lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 55 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(All lanes : Anti-HDAC1 Antibody (K464) at 1:2000 dilutionLane 1: Hela whole cell lysatesLane 2: Jurkat whole cell lysatesLane 3: K562 whole cell lysatesLane 4: mouse thymus lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 55 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Immunofluorescence (IF)
(Fluorescent image of Hela cell stained with HDAC1 Antibody (C-term). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HDAC1 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree).HDAC1 immunoreactivity is localized to Nucleus significantly.)
Immunofluorescence (IF)
(Fluorescent image of Hela cell stained with HDAC1 Antibody (C-term). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HDAC1 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree).HDAC1 immunoreactivity is localized to Nucleus significantly.)
Western Blot (WB)
(The anti-HDAC1 Pab is used in Western blot to detect HDAC1 in ZR-75-1 cell lysate.)
Western Blot (WB)
(The anti-HDAC1 Pab is used in Western blot to detect HDAC1 in ZR-75-1 cell lysate.)
Western Blot (WB)
(The anti-HDAC1 Pab was used in Western Blot to detect HDAC1 in HEK293 cells.Knockdown of HDAC1 using siRNA against HDAC1 showed a significant decrease of HDAC1 protein using this anti-HDAC1 Pab in HEK293 cells.)
Western Blot (WB)
(The anti-HDAC1 Pab was used in Western Blot to detect HDAC1 in HEK293 cells.Knockdown of HDAC1 using siRNA against HDAC1 showed a significant decrease of HDAC1 protein using this anti-HDAC1 Pab in HEK293 cells.)
Wang, S., et al., Oncogene 22(40):6204-6213 (2003).
Xia, Z.B., et al., Proc. Natl. Acad. Sci. U.S.A. 100(14):8342-8347 (2003).
Rocha, S., et al., Mol. Cell. Biol. 23(13):4713-4727 (2003).
Macaluso, M., et al., Oncogene 22(23):3511-3517 (2003).
NCBI and Uniprot Product Information
NCBI Description
Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, it deacetylates p53 and modulates its effect on cell growth and apoptosis. [provided by RefSeq, Jul 2008]
Uniprot Description
HDAC1: a transcriptional regulator of the histone deacetylase family, subfamily 1. Deacetylates lysine residues on the N-terminal part of the core histones H2A, H2B, H3 AND H4. Plays an important role in transcriptional regulation, cell cycle progression and developmental events.
Protein type: Nuclear receptor co-regulator; EC 3.5.1.98; Hydrolase
Chromosomal Location of Human Ortholog: 1p34
Cellular Component: nucleoplasm; protein complex; Sin3 complex; histone deacetylase complex; nuclear chromatin; cytoplasm; NuRD complex; chromatin; cytosol; nucleus
Molecular Function: deacetylase activity; nucleosomal DNA binding; transcription activator binding; histone deacetylase binding; protein deacetylase activity; protein N-terminus binding; transcription factor binding; protein binding; NAD-dependent histone deacetylase activity (H3-K9 specific); enzyme binding; NAD-dependent histone deacetylase activity (H3-K14 specific); NAD-dependent histone deacetylase activity (H4-K16 specific); histone deacetylase activity; transcription factor activity
Biological Process: establishment and/or maintenance of chromatin architecture; viral reproduction; nerve growth factor receptor signaling pathway; positive regulation of transcription, DNA-dependent; negative regulation of transcription from RNA polymerase II promoter; regulation of gene expression, epigenetic; negative regulation of cell cycle; protein amino acid deacetylation; negative regulation of gene expression, epigenetic; transforming growth factor beta receptor signaling pathway; positive regulation of cell proliferation; circadian regulation of gene expression; transcription initiation from RNA polymerase II promoter; Notch signaling pathway; transcription, DNA-dependent; histone deacetylation; chromatin modification; odontogenesis of dentine-containing teeth; chromatin remodeling; ATP-dependent chromatin remodeling; epidermal cell differentiation; gene expression; positive regulation of transcription from RNA polymerase II promoter; mitotic cell cycle; embryonic digit morphogenesis; blood coagulation; negative regulation of transcription, DNA-dependent; negative regulation of apoptosis
Research Articles on HDAC1
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Product Notes
The HDAC1 hdac1 (Catalog #AAA9209214) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 449-482. The HDAC1 Antibody (C-term) reacts with Human, mouse (Predicted Reactivity: Mouse) and may cross-react with other species as described in the data sheet. AAA Biotech's HDAC1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunofluorescence (IF). WB~~1:1000. Researchers should empirically determine the suitability of the HDAC1 hdac1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HDAC1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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