Rabbit LIMK2 Polyclonal Antibody | anti-LIMK2 antibody

LIMK2 Antibody (C-term)

Cat. Num. AAA9231681

• Reactivity: Human; Predicted: Bovine
• Applications: Western Blot, Immunohistochemistry, ELISA
• Synonyms: LIMK2; Polyclonal Antibody; LIMK2 Antibody (C-term); LIM domain kinase 2; anti-LIMK2 antibody

• Host: Rabbit
• Reactivity: Human; Predicted: Bovine
• Clonality: Polyclonal
• Isotype: Ig
• Specificity: This LIMK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 570-599 amino acids from the C-terminal region of human LIMK2.
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Sequence Length: 686

• Applicable Applications for anti-LIMK2 antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin, ELISA (EIA)
• Application Notes: WB: 1:1000; IHC-P: 1:25
• Antigen Source: Human
• Antigen Region: 570-599
• Antigen Type: Synthetic Peptide
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Western blot analysis of lysates from human placenta tissue and Jurkat cell line (from left to right), using LIMK2 Antibody (C-term). It was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.)

Immunohistochemistry (IHC)-Paraffin

(Immunohistochemical analysis of paraffin-embedded H.kidney section using LIMK2 Antibody (C-term) . It was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)

Related Product Information for anti-LIMK2 antibody

Description: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Background: Displays serine/threonine-specific phosphorylation of myelin basic protein and histone (MBP) in vitro.

NCBI and Uniprot Product Information

• NCBI GI #: 73390140
• NCBI GeneID: 3985
• NCBI Accession #: NP_001026971.1; NP_005560.1; NP_057952.1
• NCBI GenBank Nucleotide #: NP_001026971.1
• UniProt Accession #: P53671; Q32L23
• Molecular Weight: 72232
• NCBI Official Full Name: LIM domain kinase 2 isoform 1
• NCBI Official Synonym Full Names: LIM domain kinase 2
• NCBI Official Symbol: LIMK2
• NCBI Protein Information: LIM domain kinase 2
• UniProt Protein Name: LIM domain kinase 2
• Protein Family: LIM domain kinase
• UniProt Gene Name: LIMK2
• UniProt Synonym Gene Names: LIMK-2
• UniProt Entry Name: LIMK2_HUMAN

NCBI Description

There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. The protein encoded by this gene is phosphorylated and activated by ROCK, a downstream effector of Rho, and the encoded protein, in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. It is thought that this pathway contributes to Rho-induced reorganization of the actin cytoskeleton. At least three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

LIMK2: a TKL kinase of the LISK family that regulates actin dynamics. Contains two N-terminal LIM motifs. Phosphorylated and activated by PAK1 and ROCK, downstream effectors of Rho. Phosphorylates cofilin, inhibiting its actin-depolymerizing activity. May contributes to Rho-induced reorganization of the actin cytoskeleton. Two alternative splice variants have been identified.

Protein type: EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Protein kinase, TKL; Kinase, protein; TKL group; LISK family; LIMK subfamily

Chromosomal Location of Human Ortholog: 22q12.2

Cellular Component: cis-Golgi network; cytoplasm; nucleus

Molecular Function: protein serine/threonine kinase activity; protein binding; zinc ion binding; protein heterodimerization activity; ATP binding

Biological Process: spermatogenesis; phosphorylation; protein amino acid phosphorylation