Rabbit LIMK2 Polyclonal Antibody | anti-LIMK2 antibody

LIMK2 (LIM Domain Kinase 2, LIMK-2) (PE)

Cat. Num. AAA6424779

• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by affinity chromatography.
• Synonyms: LIMK2; Polyclonal Antibody; LIMK2 (LIM Domain Kinase 2; LIMK-2) (PE); anti-LIMK2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes LIMK2. Species Crossreactivity: human, mouse, rat
• Purity/Purification: Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
• Concentration: ~1mg/ml (varies by lot)

• Applicable Applications for anti-LIMK2 antibody: Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: IHC: 1:50-1:200; WB: 1:500-1:2000; Applications are based on unconjugated antibody.
• Immunogen: Recombinant protein corresponding to human LIMK2.
• Conjugate: PE
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using LIMK2 antibody.)

Product Categories/Family for anti-LIMK2 antibody

Antibodies; Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 73390140
• NCBI GeneID: 3985
• NCBI Accession #: NP_001026971.1
• NCBI GenBank Nucleotide #: NM_001031801.1
• UniProt Accession #: P53671; Q7KZ80; Q7L3H5; Q96E10; Q99464; Q9UFU0; A8K6H5
• Molecular Weight: 72,232 Da
• NCBI Official Full Name: LIM domain kinase 2 isoform 1
• NCBI Official Synonym Full Names: LIM domain kinase 2
• NCBI Official Symbol: LIMK2
• NCBI Protein Information: LIM domain kinase 2
• UniProt Protein Name: LIM domain kinase 2
• Protein Family: LIM domain kinase
• UniProt Gene Name: LIMK2
• UniProt Synonym Gene Names: LIMK-2
• UniProt Entry Name: LIMK2_HUMAN

NCBI Description

There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. The protein encoded by this gene is phosphorylated and activated by ROCK, a downstream effector of Rho, and the encoded protein, in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. It is thought that this pathway contributes to Rho-induced reorganization of the actin cytoskeleton. At least three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

LIMK2: a TKL kinase of the LISK family that regulates actin dynamics. Contains two N-terminal LIM motifs. Phosphorylated and activated by PAK1 and ROCK, downstream effectors of Rho. Phosphorylates cofilin, inhibiting its actin-depolymerizing activity. May contributes to Rho-induced reorganization of the actin cytoskeleton. Two alternative splice variants have been identified.

Protein type: EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Protein kinase, TKL; Kinase, protein; TKL group; LISK family; LIMK subfamily

Chromosomal Location of Human Ortholog: 22q12.2

Cellular Component: cis-Golgi network; cytoplasm; nucleus

Molecular Function: protein serine/threonine kinase activity; protein binding; zinc ion binding; protein heterodimerization activity; ATP binding

Biological Process: spermatogenesis; phosphorylation; protein amino acid phosphorylation