Western Blot (WB)
(Sample Data: Western blot using Anti-LIMK1 (1:500 dilution) in 18 ug of lysates from mouse brain. The doublet band at ~75 kDa may represent phosphorylated and non-phosphorylated forms of the protein.)
Rabbit anti-Human LIMK1 Polyclonal Antibody | anti-LIMK1 antibody
Anti-LIMK1
Reactivity
Human
Applications
ELISA, Immunofluorescence, Western Blot
Purity
Immunoaffinity chromatography
Synonyms
LIMK1; Polyclonal Antibody; Anti-LIMK1; anti-LIMK1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit, IgG
Specificity
Recognizes the human LIM Kinase protein
Purity/Purification
Immunoaffinity chromatography
Form/Format
0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH7.2 + 0.01% (w/v) Sodium Azide
Applicable Applications for anti-LIMK1 antibody
ELISA (EIA), Immunofluorescence (IF), Western Blot (WB)
Immunogen
The antibody was produced against synthesized peptide corresponding to amino acids 630-647 of human LIM Kinase protein.
Preparation and Storage
1 year at -20 degree C from date of shipment
Western Blot (WB)
(Sample Data: Western blot using Anti-LIMK1 (1:500 dilution) in 18 ug of lysates from mouse brain. The doublet band at ~75 kDa may represent phosphorylated and non-phosphorylated forms of the protein.)
Western Blot (WB)
(Sample Data: Western blot using Anti-LIMK1 (1:500 dilution) in 18 ug of lysates from mouse brain. The doublet band at ~75 kDa may represent phosphorylated and non-phosphorylated forms of the protein.)
Related Product Information for anti-LIMK1 antibody
LIMK1 or LIM domain kinase 1 contains a unique combination of 2 N-terminal LIM domain and a C-terminal protein kinase domain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers that can bind to DNA/RNA as well as mediating protein-protein interactions (1). LIMK1 is thought to be a component of an intracellular signaling pathway that may be involved in brain development especially development of nerve cells. LIMK1 may play an important role in areas of the brain that are responsible for processing visual-spatial information (visuospatial constructive cognition). LIMK1 can regulate aspects of the cytoskeleton, the structural framework that helps to determine cell shape, size, and movement (2).
Product Categories/Family for anti-LIMK1 antibody
References
1. Davila, M. et al: LIM kinase 1 is essential for the invasive growth of prostate epithelial cells: implications in prostate cancer. J Biol Chem. 2003;19;278(38):36868-75.
2. Davila, M. et al: Expression of LIM kinase 1 is associated with reversible G1/S phase arrest, chromosomal instability and prostate cancer. Mol Cancer. 2007;8;6:40.
2. Davila, M. et al: Expression of LIM kinase 1 is associated with reversible G1/S phase arrest, chromosomal instability and prostate cancer. Mol Cancer. 2007;8;6:40.
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Product Notes
The LIMK1 (Catalog #AAA516050) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-LIMK1 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's LIMK1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunofluorescence (IF), Western Blot (WB). Researchers should empirically determine the suitability of the LIMK1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LIMK1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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