Rabbit anti-Mouse LIMD1 Polyclonal Antibody | anti-LIMD1 antibody

LIMD1, CT (LIMD1, LIM domain-containing protein 1) (AP)

Cat. Num. AAA6316119

• Reactivity: Mouse
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: LIMD1; Polyclonal Antibody; CT (LIMD1; LIM domain-containing protein 1) (AP); LIM domain-containing protein 1; anti-LIMD1 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-LIMD1 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-50; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: LIMD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 437-467 amino acids from the C-terminal region of human LIMD1.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(LIMD1 Antibody (C-term) western blot analysis in mouse brain tissue lysates (35ug/lane).This demonstrates the LIMD1 antibody detected the LIMD1 protein (arrow).)

Immunohistochemistry (IHC)

(LIMD1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of LIMD1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-LIMD1 antibody

LIMD1 is a transcriptional regulator that inhibits E2F-mediated transcription, and suppresses the expression of the majority of genes with E2F1-responsive elements. May act as a tumor suppressor by inhibiting cell proliferation.

Product Categories/Family for anti-LIMD1 antibody

Antibodies; Transcription Factors

References

Ghosh, S., et al. Mol. Cancer 9, 58 (2010): Sharp, T.V., et al. Proc. Natl. Acad. Sci. U.S.A. 105(50):19932-19937(2008). Spendlove, I., et al. Int. J. Cancer 123(10):2247-2253(2008). Huggins, C.J., et al. Cancer Lett. 267(1):55-66(2008). Huggins, C.J., et al. Cancer Genet. Cytogenet. 178(1):36-41(2007).

NCBI and Uniprot Product Information

• NCBI GI #: 7657307
• NCBI GeneID: 8994
• NCBI Accession #: NP_055055.1
• NCBI GenBank Nucleotide #: NM_014240.2
• UniProt Accession #: Q9UGP4; Q17RQ1; Q9BQQ9; Q9NQ47
• Molecular Weight: 72,190 Da
• NCBI Official Full Name: LIM domain-containing protein 1
• NCBI Official Synonym Full Names: LIM domains containing 1
• NCBI Official Symbol: LIMD1
• NCBI Protein Information: LIM domain-containing protein 1
• UniProt Protein Name: LIM domain-containing protein 1
• Protein Family: LIM domain-containing protein
• UniProt Gene Name: LIMD1
• UniProt Entry Name: LIMD1_HUMAN

Uniprot Description

LIMD1: Adapter or scaffold protein which participates in the assembly of numerous protein complexes and is involved in several cellular processes such as cell fate determination, cytoskeletal organization, repression of gene transcription, cell-cell adhesion, cell differentiation, proliferation and migration. Positively regulates microRNA (miRNA)-mediated gene silencing and is essential for P-body formation and integrity. Acts as a hypoxic regulator by bridging an association between the prolyl hydroxylases and VHL enabling efficient degradation of HIF1A. Acts as a transcriptional corepressor for SNAI1- and SNAI2/SLUG- dependent repression of E-cadherin transcription. Negatively regulates the Hippo signaling pathway and antagonizes phosphorylation of YAP1. Inhibits E2F-mediated transcription, and suppresses the expression of the majority of genes with E2F1- responsive elements. Regulates osteoblast development, function, differentiation and stress osteoclastogenesis. Enhances the ability of TRAF6 to activate adapter protein complex 1 (AP-1) and negatively regulates the canonical Wnt receptor signaling pathway in osteoblasts. May act as a tumor suppressor by inhibiting cell proliferation. Belongs to the zyxin/ajuba family.

Protein type: Tumor suppressor; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 3p21.3

Cellular Component: adherens junction; focal adhesion; cytoplasm; nucleus

Molecular Function: protein binding; zinc ion binding; transcription corepressor activity

Biological Process: cell migration; transcription, DNA-dependent; multicellular organismal development; cytoskeleton organization and biogenesis; signal transduction; miRNA-mediated gene silencing; osteoblast development; regulation of cell shape; regulation of transcription, DNA-dependent; response to hypoxia; cytoplasmic mRNA processing body assembly; negative regulation of osteoblast differentiation; negative regulation of transcription, DNA-dependent; phosphorylation