Rabbit Lim3 Polyclonal Antibody | anti-Lim3 antibody

Lim3 (LHX3, P-Lim, Lim Region Containing Transcription Factor)

Cat. Num. AAA612147

• Gene Names: Lim3; CG10699; CG15174; DmelCG10699; l(2)37Bd; l(2)Bd; Lim-3A; lim3
• Reactivity: Frog, Human, Mouse, Rat
• Applications: Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry, Immunofluorescence
• Purity: Purified; Purified
• Synonyms: Lim3; Polyclonal Antibody; Lim3 (LHX3; P-Lim; Lim Region Containing Transcription Factor); Anti -Lim3 (LHX3; anti-Lim3 antibody

• Host: Rabbit
• Reactivity: Frog, Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes mouse Lim3. Does not appear to crossreact with gsh-4 on tissue sections, but may crossreact in Immunoprecipitation and Western Blot. Species Crossreactivity: human, rat, frog, fish and LIM-3 protein from whole-mount fish embryo.
• Purity/Purification: Purified; Purified
• Form/Format: Supplied as a liquid in PBS, 0.1% sodium azide.

• Applicable Applications for anti-Lim3 antibody: Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: Suitable for use in Immunohistochemistry, Western Blot, Immunoprecipitation, Immunocytochemistry and Immunofluorescence.; Dilution: Immunohistochemistry: Recommended fixative: MEMFA. Immunohistochemistry (Paraffin): 1:250-1:1000; Immunohistochemistry (Frozen): 1:3000-1:6000; Immunohistochemistry (Whole mounts): 1:4000-1:8000; Western Blot: 1:2500-1:5000; Immunoprecipitation: 1:200; Immunocytochemistry: 1:200-1:500; Immunofluorescence: Use a secondary antibody conjugated to biotin and then streptavidin-conjugated to fluorochrome.
• Immunogen: C-terminal portion of mouse Lim3.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Related Product Information for anti-Lim3 antibody

LHX3 is one of several transcription factors that have been implicated in pituitary development. LHX3 is critical for motor neuron specification and the development of the anterior and intermediate lobes of the pituitary gland in mammals. A point mutation in the LHX3 gene has recently been implicated in a severe form of combined pituitary hormone deficiency in humans. Patients who have both a missense mutation and an intragenic deletion in the LHX3 gene display retarded growth, pituitary hormone deficiency and abnormal neck and cervical spine development. Recent laboratory studies show that mutant forms of the LHX3 protein are not fully able to activate pituitary hormone gene promoters. Mice with a targeted disruption of the LHX3 gene are usually stillborn or die shortly after birth and do not possess either the anterior or intermediate lobes of the pituitary gland. The possibility exists that other mutations in the LHX3/P-LIM/LIM-3 gene may result in many additional types of pituitary/growth conditions in both humans and animals. With this new information, various assays, tests, and ultimately treatments could be developed for conditions/diseases related to mutations in the LHX3/P-LIM/LIM-3 gene.

Product Categories/Family for anti-Lim3 antibody

Antibodies; Abs to Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 24585132
• NCBI GeneID: 35184
• NCBI Accession #: NP_724161.1
• NCBI GenBank Nucleotide #: NM_165277.1
• NCBI Official Full Name: Lim3, isoform B
• NCBI Official Symbol: Lim3
• NCBI Official Synonym Symbols: CG10699; CG15174; DmelCG10699; l(2)37Bd; l(2)Bd; Lim-3A; lim3
• NCBI Protein Information: CG10699 gene product from transcript CG10699-RB; Lim3, isoform B; CG10699-PB; Lim3-PB; lethal(2)37Bd; Lim3, isoform A; CG10699-PA; Lim3-PA
• Protein Family: Protein