Rabbit Lim-1 Polyclonal Antibody | anti-LHX1 antibody

Lim-1 (Homeobox Protein Lim1, LIM Homeobox Protein 1, LHX1, LIM/Homeobox Protein Lhx1, MGC126723, MGC138141)

Cat. Num. AAA611342

• Gene Names: LHX1; LIM1; LIM-1
• Reactivity: Frog, Human, Mouse, Rat
• Applications: Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry
• Purity: Purified; Purified
• Synonyms: Lim-1; Polyclonal Antibody; Lim-1 (Homeobox Protein Lim1; LIM Homeobox Protein 1; LHX1; LIM/Homeobox Protein Lhx1; MGC126723; MGC138141); Anti -Lim-1 (Homeobox Protein Lim1; anti-LHX1 antibody

• Host: Rabbit
• Reactivity: Frog, Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes frog Lim-1. Does not crossreact with Lim-5 (the closest homolog of Lim-1 in frog) on tissue sections but does crossreact with Lim-5 in Western Blot and Immunoprecipitation.
• Purity/Purification: Purified; Purified
• Form/Format: Supplied as a liquid in 0.02M PBS, pH 7.2, 0.1% sodium azide.

• Applicable Applications for anti-LHX1 antibody: Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
• Application Notes: Suitable for use in Immunohistochemistry, Western Blot, Immunocytochemistry and Immunoprecipitation. Not suitable for Immunofluorescence.; Dilution: Immunohistochemistry (Paraffin): 1:200-1:1000 (Fish and whole mounts: 1:500) Recommended fixative MEMFA.; Western Blot: 1:3000-1:6000; Immunocytochemistry: 1:500 on P19 cell line; Immunoprecipitation: 1:200
• Immunogen: Synthetic peptide corresponding to C-terminal portion of frog Lim-1.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Testing Data

(Int. J. Dev. Biol. 42: 61 - 66 (1998) http://www.ijdb.ehu.es/web/paper.php?doi=9496787)

Related Product Information for anti-LHX1 antibody

The LIM motif defines a zinc-binding domain that is found in a variety of transcriptional regulators and proto-oncogenes products. LIM domain mediates protein-protein interactions and, with LIM-homeodomain proteins, act as negative regulators of the transcriptional activation function of the protein. This transcription factor is required for embryonic survival and for the determination of many cell types.

Product Categories/Family for anti-LHX1 antibody

Antibodies; Abs to Transcription Factors; Homeobox (HOX)

NCBI and Uniprot Product Information

• NCBI GI #: 30410785
• NCBI GeneID: 3975
• NCBI Accession #: NP_005559.2
• NCBI GenBank Nucleotide #: NM_005568.3
• UniProt Accession #: P48742; Q3MIW0
• Molecular Weight: 44,808 Da
• NCBI Official Full Name: LIM/homeobox protein Lhx1
• NCBI Official Synonym Full Names: LIM homeobox 1
• NCBI Official Symbol: LHX1
• NCBI Official Synonym Symbols: LIM1; LIM-1
• NCBI Protein Information: LIM/homeobox protein Lhx1; LIM homeobox protein 1; homeobox protein Lim-1
• UniProt Protein Name: LIM/homeobox protein Lhx1
• UniProt Gene Name: LHX1
• UniProt Synonym Gene Names: LIM-1; LIM1; LIM homeobox protein 1; hLim-1
• UniProt Entry Name: LHX1_HUMAN

NCBI Description

This gene encodes a member of a large protein family which contains the LIM domain, a unique cysteine-rich zinc-binding domain. The encoded protein is a transcription factor important for the development of the renal and urogenital systems. This gene is a candidate for Mayer-Rokitansky-Kuster-Hauser syndrome, a disorder characterized by anomalies in the female genital tract. [provided by RefSeq, Dec 2010]

Uniprot Description

LHX1: Potential transcription factor. May play a role in early mesoderm formation and later in lateral mesoderm differentiation and neurogenesis.

Protein type: Transcription factor; Cell development/differentiation

Chromosomal Location of Human Ortholog: 17q12

Cellular Component: protein complex; nucleus

Molecular Function: zinc ion binding; sequence-specific DNA binding; transcription factor activity; transcription corepressor activity

Biological Process: transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; motor axon guidance; forebrain regionalization; uterus development; embryonic pattern specification; pronephros development; post-embryonic development; anterior/posterior pattern formation; ectoderm formation; cell-cell signaling; ureteric bud development; anatomical structure formation; cerebellum development; kidney development; fallopian tube development; cervix development; nervous system development; anatomical structure morphogenesis; ventral spinal cord development; vagina development; embryonic viscerocranium morphogenesis; gastrulation with mouth forming second; somite rostral/caudal axis specification; pattern specification process; Purkinje cell-granule cell precursor cell signaling involved in regulation of granule cell precursor cell proliferation; organ morphogenesis; telencephalon development; positive regulation of embryonic development; dorsal/ventral pattern formation; ureteric bud branching; regulation of gene expression; endoderm formation; embryonic retina morphogenesis in camera-type eye; cerebellar Purkinje cell differentiation; spinal cord association neuron differentiation; negative regulation of transcription, DNA-dependent; urogenital system development; anterior/posterior axis specification