Rabbit anti-Human LIM2 Polyclonal Antibody | anti-LIM2 antibody

LIM2, CT (LIM2, Lens fiber membrane intrinsic protein, MP18, MP19, MP20) (AP)

Cat. Num. AAA6316097

• Gene Names: LIM2; MP17; MP19; CTRCT19
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: LIM2; Polyclonal Antibody; CT (LIM2; Lens fiber membrane intrinsic protein; MP18; MP19; MP20) (AP); MP20; anti-LIM2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
• Sequence Length: 215

• Applicable Applications for anti-LIM2 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: LIM2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 109-137 amino acids from the C-terminal region of human LIM2.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(LIM2 Antibody (C-term) western blot analysis in MDA-MB453 cell line lysates (35ug/lane).This demonstrates the LIM2 antibody detected the LIM2 protein (arrow).)

Related Product Information for anti-LIM2 antibody

LIM2 is an eye lens-specific protein found at the junctions of lens fiber cells, where it may contribute to cell junctional organization. It acts as a receptor for calmodulin, and may play an important role in both lens development and cataractogenesis. Mutations in this gene have been associated with cataract formation.

Product Categories/Family for anti-LIM2 antibody

Antibodies; Membrane Proteins

References

Ponnam, S.P., et al. Mol. Vis. 14, 1204-1208 (2008): Hsu, H., et al. Sheng Wu Gong Cheng Xue Bao 20(4):507-515(2004). Wistow, G., et al. Mol. Vis. 8, 171-184 (2002): Wistow, G., et al. Mol. Vis. 8, 185-195 (2002): Pras, E., et al. Am. J. Hum. Genet. 70(5):1363-1367(2002).

NCBI and Uniprot Product Information

• NCBI GI #: 21361300
• NCBI GeneID: 3982
• NCBI Accession #: NP_085915.2; NP_001155220.1
• NCBI GenBank Nucleotide #: NP_085915.2
• UniProt Accession #: P55344
• NCBI Official Full Name: lens fiber membrane intrinsic protein isoform 1
• NCBI Official Synonym Full Names: lens intrinsic membrane protein 2
• NCBI Official Symbol: LIM2
• NCBI Official Synonym Symbols: MP17; MP19; CTRCT19
• NCBI Protein Information: lens fiber membrane intrinsic protein
• UniProt Protein Name: Lens fiber membrane intrinsic protein
• Protein Family: Lens fiber membrane intrinsic protein
• UniProt Gene Name: LIM2
• UniProt Entry Name: LMIP_HUMAN

NCBI Description

This gene encodes an eye lens-specific protein found at the junctions of lens fiber cells, where it may contribute to cell junctional organization. It acts as a receptor for calmodulin, and may play an important role in both lens development and cataractogenesis. Mutations in this gene have been associated with cataract formation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Sep 2009]