Rabbit LIM Domain Kinase 1 Polyclonal Antibody | anti-Limk1 antibody

LIM Domain Kinase 1, phosphorylated (Thr508) (LIMK1, LIMK-1, LIMK)

Cat. Num. AAA629095

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: LIM Domain Kinase 1; Polyclonal Antibody; phosphorylated (Thr508) (LIMK1; LIMK-1; LIMK); Anti -LIM Domain Kinase 1; anti-Limk1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human LIM Domain Kinase 1 when phosphorylated at threonine 508.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.

• Applicable Applications for anti-Limk1 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot and Immunohistochemistry.; Dilution: Western Blot: 1:500-1:1,000; Immunohistochemistry: 1:50-1:100
• Immunogen: Synthetic phosphopeptide corresponding to human LIMK1 around the phosphorylation site of threonine 508 (R-Y-TP-V-V).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(IHC staining of paraffin-embedded human breast carcinoma using MBS629095. Negative control image on the right is tissue blocked with the phosphopeptide.)

Related Product Information for anti-Limk1 antibody

LIMK-1 belongs to a small subfamily of LIM proteins with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. LIMK1 regulates actin filament dynamics. It phoshorylates and inactivates the actin binding/ depolymerising factor cofilin, consequently stabilising the actin cytoskeleton. It may also be involved in brain development. LIMK-1 localises to the cytoplasm and is highly expressed in both the adult and fetal nervous system. It is expressed to a lesser extent in heart and skeletal muscle. LIMK-1 hemizygosity is implicated in the impaired visuospatial constructive cognition of Williams syndrome.

Product Categories/Family for anti-Limk1 antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 158186665
• NCBI GeneID: 65172
• NCBI Accession #: NP_113915.2
• NCBI GenBank Nucleotide #: NM_031727.2
• UniProt Accession #: P53669
• Molecular Weight: 72,594 Da
• NCBI Official Full Name: LIM domain kinase 1
• NCBI Official Synonym Full Names: LIM domain kinase 1
• NCBI Official Symbol: Limk1
• NCBI Protein Information: LIM domain kinase 1; LIMK-1; LIM motif-containing protein kinase 1; LIM-domain containing protein kinase 1; LIM-domain containing, protein kinase 1
• UniProt Protein Name: LIM domain kinase 1
• Protein Family: LIM domain kinase
• UniProt Gene Name: Limk1
• UniProt Synonym Gene Names: Limk
• UniProt Entry Name: LIMK1_RAT

NCBI Description

kinase that may be involved in contol of actin dynamics in the cell [RGD]

Uniprot Description

LIMK1: a TKL kinase of the LISK family which mediates Rho signaling to cytoskeleton. Contains two N-terminal LIM motifs. Phosphorylated and activated by PAK1 and ROCK, downstream effectors of Rho. May be involved in brain development. Phosphorylates and inactivates the actin binding/depolymerizing factor cofilin and induces actin cytoskeletal changes. The LIM domain interacts with the cytoplasmic domain of NRG1. Binds ROCK1. Interacts with slingshot 1. Overexpressed in prostate tumors and prostate and breast cancer cell lines; manipulation of activity correlates with invasiveness in breast and prostate cancer models. Located within the 7q11.2 amplicon associated with metastatic prostate cancer. Loss of one copy of LIMK1 is the likely cause of visuospatial cognition defects seen in small chromosomal deletions associated with dominant Williams-Beuren syndrome Three splice variant have been identified.

Protein type: Protein kinase, Ser/Thr (non-receptor); Protein kinase, TKL; Kinase, protein; EC 2.7.11.1; TKL group; LISK family; LIMK subfamily

Cellular Component: nucleoplasm; Golgi apparatus; neuron projection; focal adhesion; membrane; perinuclear region of cytoplasm; cytoplasm; nucleus

Molecular Function: protein serine/threonine kinase activity; zinc ion binding; protein heterodimerization activity; heat shock protein binding; ATP binding; protein kinase activity

Biological Process: positive regulation of axon extension; positive regulation of stress fiber formation; negative regulation of ubiquitin-protein ligase activity; positive regulation of actin filament bundle formation; protein amino acid phosphorylation