Testing Data
(Int. J. Dev. Biol. 42: 61-66 (1998))
Rabbit Lim-1 Polyclonal Antibody | anti-Lim-1 antibody
Lim-1 (Homeobox Protein Lim1, LIM Homeobox Protein 1, LHX1, LIM/Homeobox Protein Lhx1, MGC126723, MGC138141) (MaxLight 750)
Reactivity
Frog, Human, Mouse, Rat
Applications
Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Purified
Synonyms
Lim-1; Polyclonal Antibody; Lim-1 (Homeobox Protein Lim1; LIM Homeobox Protein 1; LHX1; LIM/Homeobox Protein Lhx1; MGC126723; MGC138141) (MaxLight 750); anti-Lim-1 antibody
Host
Rabbit
Reactivity
Frog, Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes frog Lim-1. Does not crossreact with Lim-5 (the closest homolog of Lim-1 in frog) on tissue sections but does crossreact with Lim-5 in Western Blot and Immunoprecipitation. Species Crossreactivity: human, rat, mouse and fish. Also reacts with Lim-1 from whole-mount fish embryo.
Purity/Purification
Purified
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight750.
Applicable Applications for anti-Lim-1 antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) Paraffin, Immunoprecipitation (IP), Western Blot (WB)
Application Notes
ICC: 1:500 on P19 cell line
IHC-P: 1:200-1:1000 (Fish and whole mounts: 1:500). Recommended fixative MEMFA.
IP: 1:200
WB: 1:3000-1:6000
Not suitable for Immunofluorescence.
Applications are based on unconjugated antibody.
IHC-P: 1:200-1:1000 (Fish and whole mounts: 1:500). Recommended fixative MEMFA.
IP: 1:200
WB: 1:3000-1:6000
Not suitable for Immunofluorescence.
Applications are based on unconjugated antibody.
Immunogen
Synthetic peptide corresponding to C-terminal portion of frog Lim-1.
Conjugate
MaxLight750
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight750 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Testing Data
(Int. J. Dev. Biol. 42: 61-66 (1998))
Testing Data
(Int. J. Dev. Biol. 42: 61-66 (1998))
Product Categories/Family for anti-Lim-1 antibody
References
1. Karavanov, A., et al., Int. J. Dev. Biol. 40: 453-462 (1996).
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Product Notes
The Lim-1 (Catalog #AAA6485156) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Lim-1 (Homeobox Protein Lim1, LIM Homeobox Protein 1, LHX1, LIM/Homeobox Protein Lhx1, MGC126723, MGC138141) (MaxLight 750) reacts with Frog, Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Lim-1 can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) Paraffin, Immunoprecipitation (IP), Western Blot (WB). ICC: 1:500 on P19 cell line IHC-P: 1:200-1:1000 (Fish and whole mounts: 1:500). Recommended fixative MEMFA. IP: 1:200 WB: 1:3000-1:6000 Not suitable for Immunofluorescence. Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the Lim-1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Lim-1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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