Western Blot (WB)
(Anti-LHPP Antibody (Center) at 1:2000 dilution + U-251 MG whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 29 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Rabbit LHPP Polyclonal Antibody | anti-LHPP antibody
LHPP Antibody (Center)
Gene Names
LHPP; HDHD2B
Reactivity
Human; Predicted Reactivity: Bovine
Applications
Western Blot, ELISA
Synonyms
LHPP; Polyclonal Antibody; LHPP Antibody (Center); Phospholysine phosphohistidine inorganic pyrophosphate phosphatase; hLHPP; 3.1.3.-; 3.6.1.1; anti-LHPP antibody
Host
Rabbit
Reactivity
Human; Predicted Reactivity: Bovine
Clonality
Polyclonal
Isotype
Ig
Specificity
This LHPP antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 84-116 amino acids from the Central region of human LHPP.
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Sequence Length
210
Applicable Applications for anti-LHPP antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB:~~1:2000
Subtitle
Purified Rabbit Polyclonal Antibody (Pab)
Cellular Location
Cytoplasm. Nucleus.
Tissue Location
Expressed in brain, and at lower levels in liver and kidney. Detected in thyroid (at protein level) Expressed in liver, kidney and moderately in brain.
Antigen Type
Synthesized Peptide
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Western Blot (WB)
(Anti-LHPP Antibody (Center) at 1:2000 dilution + U-251 MG whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 29 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(Anti-LHPP Antibody (Center) at 1:2000 dilution + U-251 MG whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 29 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-LHPP antibody
Phosphatase that hydrolyzes imidodiphosphate, 3- phosphohistidine and 6-phospholysine. Has broad substrate specificity and can also hydrolyze inorganic diphosphate, but with lower efficiency (By similarity).
Product Categories/Family for anti-LHPP antibody
References
Yokoi F.,et al.J. Biochem. 133:607-614 (2003). Ota T.,et al.Nat. Genet. 36:40-45 (2004). Deloukas P.,et al.Nature 429:375-381 (2004). Koike E.,et al.Biochem. Biophys. Res. Commun. 341:691-696 (2006).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
29165 Da
NCBI Official Full Name
phospholysine phosphohistidine inorganic pyrophosphate phosphatase isoform 2
NCBI Official Synonym Full Names
phospholysine phosphohistidine inorganic pyrophosphate phosphatase
NCBI Official Symbol
LHPP
NCBI Official Synonym Symbols
HDHD2B
NCBI Protein Information
phospholysine phosphohistidine inorganic pyrophosphate phosphatase
UniProt Protein Name
Phospholysine phosphohistidine inorganic pyrophosphate phosphatase
UniProt Gene Name
LHPP
UniProt Synonym Gene Names
hLHPP
UniProt Entry Name
LHPP_HUMAN
Uniprot Description
LHPP: Phosphatase that hydrolyzes imidodiphosphate, 3- phosphohistidine and 6-phospholysine. Has broad substrate specificity and can also hydrolyze inorganic diphosphate, but with lower efficiency. Belongs to the HAD-like hydrolase superfamily. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Energy Metabolism - oxidative phosphorylation; Hydrolase; EC 3.6.1.1; Mitochondrial
Chromosomal Location of Human Ortholog: 10q26.13
Cellular Component: cytoplasm; cytosol; nucleoplasm; nucleus
Molecular Function: inorganic diphosphatase activity; magnesium ion binding; phosphohistidine phosphatase activity; protein homodimerization activity
Biological Process: phosphate metabolic process; protein amino acid dephosphorylation; purine ribonucleoside monophosphate biosynthetic process
Research Articles on LHPP
Similar Products
Product Notes
The LHPP lhpp (Catalog #AAA9217199) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The LHPP Antibody (Center) reacts with Human; Predicted Reactivity: Bovine and may cross-react with other species as described in the data sheet. AAA Biotech's LHPP can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB:~~1:2000. Researchers should empirically determine the suitability of the LHPP lhpp for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LHPP, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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