Rabbit anti-Human, Mouse LGR5 Polyclonal Antibody | anti-LGR5 antibody

LGR5 Antibody

Cat. Num. AAA9613091

• Gene Names: LGR5; FEX; HG38; GPR49; GPR67; GRP49
• Reactivity: Human, Mouse
• Applications: Western Blot, ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
• Synonyms: LGR5; Polyclonal Antibody; LGR5 Antibody; FEX; G protein coupled receptor 49; G protein coupled receptor 67; g protein-coupled receptor fex; G-protein coupled receptor 49; G-protein coupled receptor 67; G-protein coupled receptor HG38; GPR 49; GPR 67; GPR49; GPR67; GRP 49; GRP49; HG 38; HG38; Leucine rich repeat containing G protein coupled receptor 5; Leucine-rich repeat-containing G-protein coupled receptor 5; LGR 5; LGR5_HUMAN; MGC117008; Orphan G protein coupled receptor HG38; anti-LGR5 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Specificity: LGR5 Antibody detects endogenous levels of total LGR5.; Tissue Specificity: Expressed in skeletal muscle, placenta, spinal cord, and various region of brain. Expressed at the base of crypts in colonic and small mucosa stem cells. In premalignant cancer expression is not restricted to the cript base. Overexpressed in cancers of the ovary, colon and liver.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
• Form/Format: Liquid. Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-LGR5 antibody: Western Blot (WB), Peptide ELISA (EIA)
• Application Notes: WB: 1:500-1:1000; Peptide ELISA: 1:20,000-1:40,000
• Immunogen: A synthesized peptide derived from human LGR5 (GPR49), corresponding to a region within C-terminal amino acids.
• Conjugation: Unconjugated
• Fragment: Fab fragment
• Subunit Structure: Identified in a complex composed of RNF43, LGR5 and RSPO1. Also interacts with other R-spondin ligands, including RSPO2, RSPO3 and RSPO4.
• Similarity: Belongs to the G-protein coupled receptor 1 family.
• Subcellular Location: Cell membrane>Multi-pass membrane protein. Golgi apparatus>trans-Golgi network membrane>Multi-pass membrane protein.; Note: Rapidly and constitutively internalized to the trans-Golgi network at steady state. Internalization to the trans-Golgi network may be the result of phosphorylation at Ser-861 and Ser-864; however, the phosphorylation event has not been proven (PubMed:23439653).
• Preparation and Storage: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Western Blot (WB)

(Western blot analysis of extracts from Hela, 3T3 and HepG2 cells using LGR5(GPR49) Antibody)

Related Product Information for anti-LGR5 antibody

Receptor for R-spondins that potentiates the canonical Wnt signaling pathway and acts as a stem cell marker of the intestinal epithelium and the hair follicle. Upon binding to R-spondins (RSPO1, RSPO2, RSPO3 or RSPO4), associates with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. In contrast to classical G-protein coupled receptors, does not activate heterotrimeric G-proteins to transduce the signal. Involved in the development and/or maintenance of the adult intestinal stem cells during postembryonic development.
Orphan receptor. Stem cell marker of the intestinal epithelium and the hair follicule. Target gene of Wnt signaling.

NCBI and Uniprot Product Information

• NCBI GI #: 469608414
• NCBI GeneID: 8549
• NCBI Accession #: NP_001264155.1
• NCBI GenBank Nucleotide #: NM_001277226.1
• UniProt Accession #: O75473; Q4VAM0; Q4VAM2; Q9UP75; D8MCT0
• Molecular Weight: 99998 MW
• NCBI Official Full Name: leucine-rich repeat-containing G-protein coupled receptor 5 isoform 2
• NCBI Official Synonym Full Names: leucine rich repeat containing G protein-coupled receptor 5
• NCBI Official Symbol: LGR5
• NCBI Official Synonym Symbols: FEX; HG38; GPR49; GPR67; GRP49
• NCBI Protein Information: leucine-rich repeat-containing G-protein coupled receptor 5
• UniProt Protein Name: Leucine-rich repeat-containing G-protein coupled receptor 5
• Protein Family: Leucine-rich repeat-containing G-protein coupled receptor
• UniProt Gene Name: LGR5
• UniProt Synonym Gene Names: GPR49; GPR67

NCBI Description

The protein encoded by this gene is a leucine-rich repeat-containing receptor (LGR) and member of the G protein-coupled, 7-transmembrane receptor (GPCR) superfamily. The encoded protein is a receptor for R-spondins and is involved in the canonical Wnt signaling pathway. This protein plays a role in the formation and maintenance of adult intestinal stem cells during postembryonic development. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2015]

Uniprot Description

Receptor for R-spondins that potentiates the canonical Wnt signaling pathway and acts as a stem cell marker of the intestinal epithelium and the hair follicle. Upon binding to R-spondins (RSPO1, RSPO2, RSPO3 or RSPO4), associates with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. In contrast to classical G-protein coupled receptors, does not activate heterotrimeric G-proteins to transduce the signal. Involved in the development and/or maintenance of the adult intestinal stem cells during postembryonic development.