Rabbit LGR4 Polyclonal Antibody | anti-LGR4 antibody

LGR4 Polyclonal Antibody

Cat. Num. AAA3010382

• Gene Names: LGR4; GPR48; BNMD17
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: LGR4; Polyclonal Antibody; LGR4 Polyclonal Antibody; BNMD17; GPR48; anti-LGR4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: LGR4 polyclonal antibody detects endogenous levels of LGR4 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 951

• Applicable Applications for anti-LGR4 antibody: Western Blot (WB), Immunofluorescence (IF)
• Application Notes: WB: 1:1000-1:3000; IF: 1:50-1:200
• Immunogen: Recombinant protein of human LGR4
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot analysis of extracts of various cell lines, using LGR4 antibody.)

Related Product Information for anti-LGR4 antibody

Receptor for R-spondins that potentiates the canonical Wnt signaling pathway and is involved in the formation of various organs. Upon binding to R-spondins (RSPO1, RSPO2, RSPO3 or RSPO4), associates with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. In contrast to classical G-protein coupled receptors, does not activate heterotrimeric G-proteins to transduce the signal. Its function as activator of the Wnt signaling pathway is required for the development of various organs, including liver, kidney, intestine, bone, reproductive tract and eye. May also act as a receptor for norrin (NDP), such results however require additional confirmation in vivo. Required during spermatogenesis to activate the Wnt signaling pathway in peritubular myoid cells.

NCBI and Uniprot Product Information

• NCBI GI #: 157694513
• NCBI GeneID: 55366
• NCBI Accession #: NP_060960.2
• NCBI GenBank Nucleotide #: NP_060960.2
• UniProt Accession #: Q9BXB1
• Molecular Weight: ~ 109kDa
• NCBI Official Full Name: leucine-rich repeat-containing G-protein coupled receptor 4 isoform 1
• NCBI Official Synonym Full Names: leucine rich repeat containing G protein-coupled receptor 4
• NCBI Official Symbol: LGR4
• NCBI Official Synonym Symbols: GPR48; BNMD17
• NCBI Protein Information: leucine-rich repeat-containing G-protein coupled receptor 4
• UniProt Protein Name: Leucine-rich repeat-containing G-protein coupled receptor 4
• Protein Family: Leucine-rich repeat-containing G-protein coupled receptor
• UniProt Gene Name: LGR4
• UniProt Synonym Gene Names: GPR48
• UniProt Entry Name: LGR4_HUMAN

NCBI Description

The protein encoded by this gene is a G-protein coupled receptor that binds R-spondins and activates the Wnt signaling pathway. This Wnt signaling pathway activation is necessary for proper development of many organs of the body. [provided by RefSeq, Oct 2016]

Uniprot Description

LGR4: a leucine-rich repeat-containing G-protein-coupled receptor. Receptor for R-spondins, potent stimulators of adult stem cell proliferation. A Wnt receptor component that enhances Wnt signaling by soluble R-spondin proteins. Plays an essential role during homeostasis of the adult mouse intestine.

Protein type: GPCR, family 1; Receptor, GPCR; Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 11p14-p13

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: G-protein coupled receptor activity; protein binding; transmembrane receptor activity

Biological Process: osteoblast differentiation; G-protein coupled receptor protein signaling pathway; epithelial cell proliferation; positive regulation of transcription, DNA-dependent; negative regulation of cytokine secretion; male genitalia development; negative regulation of toll-like receptor signaling pathway; innate immune response; spermatogenesis; circadian regulation of gene expression; bone remodeling; negative regulation of transcription, DNA-dependent; bone mineralization

Disease: Bone Mineral Density Quantitative Trait Locus 17