Rabbit LGMN Polyclonal Antibody | anti-LGMN antibody

LGMN Polyclonal Antibody

Cat. Num. AAA9131059

• Gene Names: LGMN; AEP; LGMN1; PRSC1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunofluorescence
• Purity: Affinity Purification
• Synonyms: LGMN; Polyclonal Antibody; LGMN Polyclonal Antibody; AEP; LGMN1; PRSC1; legumain; anti-LGMN antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity Purification
• Form/Format: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

• Applicable Applications for anti-LGMN antibody: Western Blot (WB), Immunofluorescence (IF)
• Application Notes: Recommended Dilutions: WB: 1:1000 - 1:2000, IF: 1:50 - 1:200
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 18-323 of human LGMN (NP_001008530.1).
• Preparation and Storage: Store at -20 degree C. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using LGMN antibody (MBS9131059) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.)

Immunofluorescence (IF)

(Immunofluorescence analysis of C6 cells using LGMN Polyclonal Antibody (MBS9131059) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)

Immunofluorescence (IF)

(Immunofluorescence analysis of L929 cells using LGMN Polyclonal Antibody (MBS9131059) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)

Immunofluorescence (IF)

(Immunofluorescence analysis of HeLa cells using LGMN Polyclonal Antibody (MBS9131059) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)

Related Product Information for anti-LGMN antibody

This gene encodes a cysteine protease that has a strict specificity for hydrolysis of asparaginyl bonds. This enzyme may be involved in the processing of bacterial peptides and endogenous proteins for MHC class II presentation in the lysosomal/endosomal systems. Enzyme activation is triggered by acidic pH and appears to be autocatalytic. Protein expression occurs after monocytes differentiate into dendritic cells. A fully mature, active enzyme is produced following lipopolysaccharide expression in mature dendritic cells. Overexpression of this gene may be associated with the majority of solid tumor types. This gene has a pseudogene on chromosome 13. Several alternatively spliced transcript variants have been described, but the biological validity of only two has been determined. These two variants encode the same isoform.

Product Categories/Family for anti-LGMN antibody

Primary antibody

NCBI and Uniprot Product Information

• NCBI GI #: 2842759
• NCBI GeneID: 5641
• UniProt Accession #: Q99538; O00123; Q86TV2; Q86TV3; Q9BTY1
• Molecular Weight: Observed MW: 54kDa; Calculated MW: 42kDa/49kDa
• NCBI Official Full Name: Legumain
• NCBI Official Synonym Full Names: legumain
• NCBI Official Symbol: LGMN
• NCBI Official Synonym Symbols: AEP; LGMN1; PRSC1
• NCBI Protein Information: legumain
• UniProt Protein Name: Legumain
• UniProt Gene Name: LGMN
• UniProt Synonym Gene Names: PRSC1

NCBI Description

This gene encodes a cysteine protease that has a strict specificity for hydrolysis of asparaginyl bonds. This enzyme may be involved in the processing of bacterial peptides and endogenous proteins for MHC class II presentation in the lysosomal/endosomal systems. Enzyme activation is triggered by acidic pH and appears to be autocatalytic. Protein expression occurs after monocytes differentiate into dendritic cells. A fully mature, active enzyme is produced following lipopolysaccharide expression in mature dendritic cells. Overexpression of this gene may be associated with the majority of solid tumor types. This gene has a pseudogene on chromosome 13. Several alternatively spliced transcript variants have been described, but the biological validity of only two has been determined. These two variants encode the same isoform. [provided by RefSeq, Jul 2008]

Uniprot Description

LGMN: Has a strict specificity for hydrolysis of asparaginyl bonds. Can also cleave aspartyl bonds slowly, especially under acidic conditions. May be involved in the processing of proteins for MHC class II antigen presentation in the lysosomal/endosomal system. Belongs to the peptidase C13 family.

Protein type: EC 3.4.22.34; Protease

Chromosomal Location of Human Ortholog: 14q32.12

Cellular Component: lysosomal lumen; lysosome

Molecular Function: cysteine-type endopeptidase activity; peptidase activity

Biological Process: antigen processing and presentation of exogenous peptide antigen via MHC class II; negative regulation of neuron apoptosis; proteolysis; proteolysis involved in cellular protein catabolic process; receptor catabolic process; renal system process; toll-like receptor signaling pathway; vacuolar protein processing; vitamin D metabolic process