Rabbit LGI4 Polyclonal Antibody | anti-LGI4 antibody

LGI4 Antibody

Cat. Num. AAA150692

• Gene Names: LGI4; LGIL3
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot
• Purity: LGI4 Antibody is affinity chromatography purified via peptide column.
• Synonyms: LGI4; Polyclonal Antibody; LGI4 Antibody; LGIL3; UNQ6515/PRO21485; Leucine-rich repeat LGI family member 4; LGI1-like protein 3; leucine-rich repeat LGI family; member 4; anti-LGI4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Two isoforms of LGI4 are known to exist; this LGI4 antibody will recognize only the larger form. This LGI4 antibody is predicted to be specific to LGI4 and not recognize other LGI proteins.
• Purity/Purification: LGI4 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 537

• Applicable Applications for anti-LGI4 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: LGI4 antibody can be used for the detection of LGI4 by Western blot at 1 - 2 mug/mL.
• Conjugate: Unconjugated
• Immunogen: LGI4 antibody was raised against a 17 amino acid synthetic peptide from near the carboxy terminus of human LGI4.
• Buffer: LGI4 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: LGI4 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of LGI4 in rat brain tissue lysate with LGI4 antibody at (A) 1 and (B) 2 μg/mL.)

Related Product Information for anti-LGI4 antibody

LGI4 Antibody: The leucine-rich, glioma inactivated gene 4 (LGI4) is a member of the LGI family in which LGI1 is the exemplar. The LGI family consists of four of highly related proteins containing leucine-rich repeats (LRRs) which are highly similar to other transmembrane signaling molecules and receptors. LGI1 has been identified as a candidate tumor suppressor gene for glioma and plays a role in autodominant lateral temporal epilepsy (ADTLE), an epileptic syndrome characterized by focal seizures with predominant auditory symptoms. Despite its high homology with LGI1 and similar pattern of expression, mutations in LGI4 have not been found to be associated with ADTLE. However, the LGI4 gene is located in a region linked to benign familial infantile convulsions. Further study revealed that a GC-to-AT polymorphism was correlated with childhood absence epilepsy. Other studies showed that decreasing LGI4 expression in cultured cells inhibits myelination, indicating that LGI4 may play a role in neural development.

NCBI and Uniprot Product Information

• NCBI GI #: 21359654
• NCBI GeneID: 163175
• UniProt Accession #: Q8N135; Q5M8T1; B2RN53; B9EGS7
• Molecular Weight: 28,231 Da
• NCBI Official Full Name: LGI4
• NCBI Official Synonym Full Names: leucine-rich repeat LGI family, member 4
• NCBI Official Symbol: LGI4
• NCBI Official Synonym Symbols: LGIL3
• NCBI Protein Information: leucine-rich repeat LGI family member 4; LGI1-like protein 3; leucine-rich glioma-inactivated gene 4; leucine-rich glioma-inactivated protein 4
• UniProt Protein Name: Leucine-rich repeat LGI family member 4
• Protein Family: Leucine-rich repeat LGI family
• UniProt Gene Name: LGI4
• UniProt Synonym Gene Names: LGIL3
• UniProt Entry Name: LGI4_HUMAN

Uniprot Description

LGI4: 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 19q13.12|19q13.11

Cellular Component: extracellular space

Biological Process: glial cell proliferation; adult locomotory behavior; neuron maturation; myelination in the peripheral nervous system