Western Blot (WB)
(Western Blot analysis in HT-29 whole cell lysate (20ug) using MBS6006042 (1:1000). Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10,000 dilution. Predicted band size: 36kD. Blocking/Dilution buffer: 5% NFDM/TBST.)
Rabbit anti-Human LGALS4 Polyclonal Antibody | anti-LGALS4 antibody
LGALS4, NT (LGALS4, Galectin-4, Antigen NY-CO-27, L-36 lactose-binding protein, Lactose-binding lectin 4) (MaxLight 405)
Gene Names
LGALS4; GAL4; L36LBP
Reactivity
Human
Applications
Western Blot, Flow Cytometry, Functional Assay
Purity
Purified by Protein A and Peptide Affinity Chromatography.
Synonyms
LGALS4; Polyclonal Antibody; NT (LGALS4; Galectin-4; Antigen NY-CO-27; L-36 lactose-binding protein; Lactose-binding lectin 4) (MaxLight 405); Lactose-binding lectin 4; anti-LGALS4 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human LGALS4.
Purity/Purification
Purified by Protein A and Peptide Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight405.
Applicable Applications for anti-LGALS4 antibody
Western Blot (WB), Flow Cytometry (FC/FACS), FLISA
Application Notes
Applications are based on unconjugated antibody.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 70-98 in the N-terminal region of human LGALS4, UniProt Accession #NP_006140.1; P56470.
Conjugate
MaxLight405
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight405 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(Western Blot analysis in HT-29 whole cell lysate (20ug) using MBS6006042 (1:1000). Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10,000 dilution. Predicted band size: 36kD. Blocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(Western Blot analysis in HT-29 whole cell lysate (20ug) using MBS6006042 (1:1000). Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10,000 dilution. Predicted band size: 36kD. Blocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(Western Blot analysis in Jurkat cell line lysates (35ug/lane) usingMBS6006042. This demonstrates that MBS6006042 detected the LGALS4 protein (arrow).)
Western Blot (WB)
(Western Blot analysis in Jurkat cell line lysates (35ug/lane) usingMBS6006042. This demonstrates that MBS6006042 detected the LGALS4 protein (arrow).)
Flow Cytometry (FC/FACS)
(Flow Cytometric analysis of Jurkat cells using MBS6006042 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(Flow Cytometric analysis of Jurkat cells using MBS6006042 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-LGALS4 antibody
The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. The expression of this gene is restricted to small intestine, colon, and rectum, and it is underexpressed in colorectal cancer.
Product Categories/Family for anti-LGALS4 antibody
References
1. Zimbardi, A.L., et al. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 66 (PT 5), 542-545 (2010). 2. Stechly, L., et al. Traffic 10(4):438-450(2009). 3. Tripodi, D., et al. BMC Med Genomics 2, 65 (2009). 4. Oberg, C.T., et al. Bioorg. Med. Chem. Lett. 18(13):3691-3694(2008). 5. Ideo, H., et al. J. Biol. Chem. 282(29):21081-21089(2007).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
35,941 Da
NCBI Official Full Name
galectin-4
NCBI Official Synonym Full Names
lectin, galactoside-binding, soluble, 4
NCBI Official Symbol
LGALS4
NCBI Official Synonym Symbols
GAL4; L36LBP
NCBI Protein Information
galectin-4; gal-4; galectin 4; antigen NY-CO-27; lactose-binding lectin 4; L-36 lactose-binding protein
UniProt Protein Name
Galectin-4
Protein Family
UniProt Gene Name
LGALS4
UniProt Synonym Gene Names
Gal-4; L36LBP
UniProt Entry Name
LEG4_HUMAN
NCBI Description
The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. The expression of this gene is restricted to small intestine, colon, and rectum, and it is underexpressed in colorectal cancer. [provided by RefSeq, Jul 2008]
Uniprot Description
galectin-4: Galectin that binds lactose and a related range of sugars. May be involved in the assembly of adherens junctions.
Chromosomal Location of Human Ortholog: 19q13.2
Cellular Component: plasma membrane; cytosol
Molecular Function: carbohydrate binding
Biological Process: cell adhesion
Research Articles on LGALS4
Similar Products
Product Notes
The LGALS4 lgals4 (Catalog #AAA6315816) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The LGALS4, NT (LGALS4, Galectin-4, Antigen NY-CO-27, L-36 lactose-binding protein, Lactose-binding lectin 4) (MaxLight 405) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's LGALS4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Flow Cytometry (FC/FACS), FLISA. Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the LGALS4 lgals4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LGALS4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
