Rabbit anti-Human Leucine-rich alpha-2-glycoprotein Polyclonal Antibody | anti-LRG antibody

Leucine-rich alpha-2-glycoprotein (LRG, HMFT1766, Leucine-rich alpha-2-glycoprotein 1, LRG1) (PE)

Cat. Num. AAA6484981

• Gene Names: LRG1; LRG; HMFT1766
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: Leucine-rich alpha-2-glycoprotein; Polyclonal Antibody; Leucine-rich alpha-2-glycoprotein (LRG; HMFT1766; Leucine-rich alpha-2-glycoprotein 1; LRG1) (PE); anti-LRG antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human LRG1.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-LRG antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:50-1:100; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the center region of human LRG1 (KLH).
• Conjugate: PE
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of LRG1 antibody (Center) in 293 cell line lysates (35ug/lane). LRG1 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human lung carcinoma reacted with LRG1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(LRG1 Antibody (Center) flow cytometry analysis of 293 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Western Blot (WB)

(Western blot analysis of LRG1 (arrow) using rabbit polyclonal LRG1 Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the LRG1 gene.)

Product Categories/Family for anti-LRG antibody

Antibodies; Carbohydrates; Glycoproteins

References

1. Shirai, R., Biochem. Biophys. Res. Commun. 382(4), 776-779 (2009). 2. Gillott, D.J., Fertil. Steril. 90(3), 761-768 (2008).

NCBI and Uniprot Product Information

• NCBI GI #: 16418467
• NCBI GeneID: 116844
• NCBI Accession #: NP_443204
• NCBI GenBank Nucleotide #: NM_052972.2
• UniProt Accession #: P02750; Q8N4F5; Q96QZ4
• Molecular Weight: 36 kDa (335aa)
• NCBI Official Full Name: leucine-rich alpha-2-glycoprotein
• NCBI Official Synonym Full Names: leucine rich alpha-2-glycoprotein 1
• NCBI Official Symbol: LRG1
• NCBI Official Synonym Symbols: LRG; HMFT1766
• NCBI Protein Information: leucine-rich alpha-2-glycoprotein
• UniProt Protein Name: Leucine-rich alpha-2-glycoprotein
• Protein Family: Leucine-rich alpha-2-glycoprotein
• UniProt Gene Name: LRG1
• UniProt Synonym Gene Names: LRG; LRG
• UniProt Entry Name: A2GL_HUMAN

NCBI Description

The leucine-rich repeat (LRR) family of proteins, including LRG1, have been shown to be involved in protein-protein interaction, signal transduction, and cell adhesion and development. LRG1 is expressed during granulocyte differentiation (O'Donnell et al., 2002 [PubMed 12223515]).[supplied by OMIM, Mar 2008]

Uniprot Description

LRG1:

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: extracellular space; membrane; extracellular region

Molecular Function: transforming growth factor beta receptor binding

Biological Process: positive regulation of angiogenesis; positive regulation of transforming growth factor beta receptor signaling pathway; brown fat cell differentiation; positive regulation of endothelial cell proliferation