Goat LEPR / Leptin Receptor Polyclonal Antibody | anti-LEPR antibody

Goat Polyclonal to Human LEPR / Leptin Receptor

Cat. Num. AAA242542

• Gene Names: LEPR; OBR; OB-R; CD295; LEP-R; LEPRD
• Reactivity: Gorilla, Human; Predicted Reactivity: Monkey, Dog, Horse (at least 90% immunogen sequence identity)
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: LEPR / Leptin Receptor; Polyclonal Antibody; Goat Polyclonal to Human LEPR / Leptin Receptor; Anti-LEPR / Leptin Receptor Antibody (Internal) IHC-plus; LEPR; CD295 antigen; HuB219; LEP-R; OB receptor; LEPRD; OB-R; OBR; CD295; DB; Leptin receptor; Human LEPR; Leptin Receptor; anti-LEPR antibody

• Host: Goat
• Reactivity: Gorilla, Human; Predicted Reactivity: Monkey, Dog, Horse (at least 90% immunogen sequence identity)
• Clonality: Polyclonal
• Specificity: Human LEPR / Leptin Receptor. This antibody is expected to recognise all three reported isoforms (NP_002294.2; NP_001003679.1; NP_001003680.1).
• Purity/Purification: Immunoaffinity Purified
• Form/Format: Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 1165

• Applicable Applications for anti-LEPR antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:16000), IHC-P (4 ug/ml), WB (1 - 3 ug/ml); Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
• Target Species: Human
• Immunogen Description: Synthetic peptide C-TQDDIEKHQSDAG from an internal region of human LEPR / Leptin Receptor (NP_002294.2; NP_001003679.1; NP_001003680.1). Percent identity by BLAST analysis: Human, Gorilla (100%); Marmoset, Panda, Dog, Horse (92%).
• Immunogen Type: Synthetic peptide
• Immunogen: LEPR / Leptin Receptor antibody was raised against synthetic peptide C-TQDDIEKHQSDAG from an internal region of human LEPR / Leptin Receptor (NP_002294.2; NP_001003679.1; NP_001003680.1). Percent identity by BLAST analysis: Human, Gorilla (100%); Marmoset, Panda, Dog, Horse (92%).
• Antigen Modification: Internal
• Preparation and Storage: Store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

(Anti-Leptin Receptor antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 4 ug/ml.)

Immunohistochemistry (IHC)

(Anti-Leptin Receptor antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 4 ug/ml.)

Western Blot (WB)

(Western blot of human brain lysate)

NCBI and Uniprot Product Information

• NCBI GI #: 40254464
• NCBI GeneID: 3953
• NCBI Accession #: NP_002294.2
• NCBI GenBank Nucleotide #: NM_002303.5
• UniProt Accession #: P48357; Q13592; Q13593; Q13594; Q92919; Q92920; Q92921
• Molecular Weight: 96,042 Da
• NCBI Official Full Name: leptin receptor isoform 1
• NCBI Official Synonym Full Names: leptin receptor
• NCBI Official Symbol: LEPR
• NCBI Official Synonym Symbols: OBR; OB-R; CD295; LEP-R; LEPRD
• NCBI Protein Information: leptin receptor; huB219; OB receptor
• UniProt Protein Name: Leptin receptor
• Protein Family: Leptin receptor
• UniProt Gene Name: LEPR
• UniProt Synonym Gene Names: DB; OBR; LEP-R; OB-R
• UniProt Entry Name: LEPR_HUMAN

NCBI Description

The protein encoded by this gene belongs to the gp130 family of cytokine receptors that are known to stimulate gene transcription via activation of cytosolic STAT proteins. This protein is a receptor for leptin (an adipocyte-specific hormone that regulates body weight), and is involved in the regulation of fat metabolism, as well as in a novel hematopoietic pathway that is required for normal lymphopoiesis. Mutations in this gene have been associated with obesity and pituitary dysfunction. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. It is noteworthy that this gene and LEPROT gene (GeneID:54741) share the same promoter and the first 2 exons, however, encode distinct proteins (PMID:9207021).[provided by RefSeq, Nov 2010]

Uniprot Description

LEPR: the receptor for leptin (obesity factor). A single-transmembrane-domain receptor of the cytokine receptor family. On ligand binding, mediates signaling through JAK2/STAT3. Involved in the regulation of fat metabolism and, in a hematopoietic pathway, required for normal lymphopoiesis. May play a role in reproduction. Five alternatively spliced transcript variants have been reported.

Protein type: Membrane protein, integral; Receptor, cytokine

Chromosomal Location of Human Ortholog: 1p31

Cellular Component: integral to membrane; plasma membrane; extracellular region; receptor complex

Molecular Function: hematopoietin/interferon-class (D200-domain) cytokine receptor activity; identical protein binding; protein binding; transmembrane receptor activity

Biological Process: cholesterol metabolic process; cell surface receptor linked signal transduction; multicellular organismal development; negative regulation of hydrolase activity; negative regulation of gluconeogenesis; cytokine and chemokine mediated signaling pathway; energy reserve metabolic process

Disease: Leptin Receptor Deficiency