Human Lectin, Mannose Binding 2 (LMAN2) ELISA Kit | LMAN2 elisa kit

Human Lectin, Mannose Binding 2 (LMAN2) ELISA Kit

Cat. Num. AAA451356

• Gene Names: LMAN2; GP36B; VIP36; C5orf8
• Reactivity: Human
• Synonyms: Lectin; Mannose Binding 2 (LMAN2); Human Lectin; Mannose Binding 2 (LMAN2) ELISA Kit; GP36B; VIP36; C5orf8; Vesicular integral-membrane protein VIP36; Glycoprotein GP36b; Vesicular integral-membrane protein 36; LMAN2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of LMAN2. No significant cross-reactivity or interference between LMAN2 and analogues was observed.
• Sequence Length: 356

• Samples: Serum, plasma, tissue homogenates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.055ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level LMAN2 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level LMAN2 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for LMAN2 elisa kit

Intended Uses: This kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of LMAN2 in human serum, plasma, tissue homogenates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to LMAN2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to LMAN2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain LMAN2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of LMAN2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 5803023
• NCBI GeneID: 10960
• NCBI Accession #: NP_006807.1
• NCBI GenBank Nucleotide #: NM_006816.2
• UniProt Accession #: Q12907; Q53HH1
• Molecular Weight: 40,229 Da
• NCBI Official Full Name: vesicular integral-membrane protein VIP36
• NCBI Official Synonym Full Names: lectin, mannose binding 2
• NCBI Official Symbol: LMAN2
• NCBI Official Synonym Symbols: GP36B; VIP36; C5orf8
• NCBI Protein Information: vesicular integral-membrane protein VIP36
• UniProt Protein Name: Vesicular integral-membrane protein VIP36
• Protein Family: Vesicular integral-membrane protein
• UniProt Gene Name: LMAN2
• UniProt Synonym Gene Names: C5orf8; VIP36

NCBI Description

This gene encodes a type I transmembrane lectin that shuttles between the endoplasmic reticulum, the Golgi apparatus and the plasma membrane. The encoded protein binds high mannose type glycoproteins and may facilitate their sorting, trafficking and quality control. [provided by RefSeq, Oct 2008]

Uniprot Description

Plays a role as an intracellular lectin in the early secretory pathway. Interacts with N-acetyl-D-galactosamine and high-mannose type glycans and may also bind to O-linked glycans. Involved in the transport and sorting of glycoproteins carrying high mannose-type glycans ().