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Western Blot (WB) (Western Blot analysis of extracts of mouse liver, using LECT1 antibody.)

Rabbit anti-Human, Mouse LECT1 Polyclonal Antibody | anti-LECT1 antibody

LECT1 Polyclonal Antibody

Reactivity
Human, Mouse
Applications
Western Blot, Immunofluorescence
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
LECT1; Polyclonal Antibody; LECT1 Polyclonal Antibody; Leukocyte cell-derived chemotaxin 1; Chondromodulin-I; ChM-I; CHMI; anti-LECT1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
LECT1 polyclonal antibody detects endogenous levels of LECT1 protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
334
Applicable Applications for anti-LECT1 antibody
Western Blot (WB), Immunofluorescence (IF)
Application Notes
WB: 1:500-1:2000
IF: 1:50-1:100
Immunogen
Recombinant full length Human LECT1.
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot analysis of extracts of mouse liver, using LECT1 antibody.)

Western Blot (WB) (Western Blot analysis of extracts of mouse liver, using LECT1 antibody.)

Immunofluorescence (IF)

(Immunofluorescence analysis of A549 cell using LECT1 antibody. Blue: DAPI for nuclear staining.)

Immunofluorescence (IF) (Immunofluorescence analysis of A549 cell using LECT1 antibody. Blue: DAPI for nuclear staining.)
Related Product Information for anti-LECT1 antibody
ChM-1 is a cartilage-specific matrix glycoprotein that stimulates the growth of chondrocytes. ChM-1 inhibits angiogenesis by disrupting the tube formation of endothelial cells and thus is responsible for the avascular nature of cartilage. ChM-1 is strongly expressed by the proliferating and hypertrophic zones in the epiphyseal plate of long bones. ChM-1 accumulates in the interterritorial matrix around the lacunae. During development, downregulation of ChM-1 permits angiogenesis and ultimately bone formation on the cartilage template. ChM-1 expression is downregulated in the presence of several growth factors including TGFbeta2, FGF2 and PTHLH. ChM-1 expression may also play a role in the hypovascularity and chondroid formation of pleomorphic adenomas. The gene encoding human ChM-1 maps to chromosome 13q14-q21.

NCBI and Uniprot Product Information

NCBI GI #
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
~ 37kDa
NCBI Official Full Name
leukocyte cell-derived chemotaxin 1 isoform 1
UniProt Protein Name
Leukocyte cell-derived chemotaxin 1
UniProt Gene Name
LECT1
UniProt Synonym Gene Names
CHMI; CH-SP; ChM-I
UniProt Entry Name
LECT1_HUMAN

Uniprot Description

LECT1: Bifunctional growth regulator that stimulates the growth of cultured chondrocytes in the presence of basic fibroblast growth factor (FGF) but inhibits the growth of cultured vascular endothelial cells. May contribute to the rapid growth of cartilage and vascular invasion prior to the replacement of cartilage by bone during endochondral bone development. Inhibits in vitro tube formation and mobilization of endothelial cells. Plays a role as antiangiogenic factor in cardiac valves to suppress neovascularization. Belongs to the chondromodulin-1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell development/differentiation; Membrane protein, integral

Chromosomal Location of Human Ortholog: 13q14.3

Cellular Component: proteinaceous extracellular matrix; integral to membrane; endomembrane system

Biological Process: proteoglycan metabolic process; negative regulation of angiogenesis; negative regulation of vascular endothelial growth factor receptor signaling pathway; cartilage development; negative regulation of endothelial cell proliferation; endothelial cell morphogenesis; skeletal development

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Product Notes

The LECT1 lect1 (Catalog #AAA3011645) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The LECT1 Polyclonal Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's LECT1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF). WB: 1:500-1:2000 IF: 1:50-1:100. Researchers should empirically determine the suitability of the LECT1 lect1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LECT1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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