Goat anti-Human, Mouse LDHC Polyclonal Antibody | anti-LDHC antibody

LDHC (L-lactate Dehydrogenase C Chain, LDH-C, Cancer/Testis Antigen 32, CT32, LDH Testis Subunit, LDH-X, LDH3, LDHX)

Cat. Num. AAA6006345

• Gene Names: LDHC; CT32; LDH3; LDHX
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: LDHC; Polyclonal Antibody; LDHC (L-lactate Dehydrogenase C Chain; LDH-C; Cancer/Testis Antigen 32; CT32; LDH Testis Subunit; LDH-X; LDH3; LDHX); Anti -LDHC (L-lactate Dehydrogenase C Chain; anti-LDHC antibody

• Host: Goat
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Specificity: Recognizes mouse LDHC. Species sequence homology: Human.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.

• Applicable Applications for anti-LDHC antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blot and Immunohistochemistry.; Dilution: ELISA: 1:16,000; Western Blot: 0.03-0.1ug/ml, observed in mouse testis lysates on ~30-35kD bands; Immunohistochemistry: Seminiferous tubules
• Immunogen: Synthetic peptide corresponding to C-DSDKEHWKNVHKQ, from mouse LDHC, at the internal region of the protein (NP_002292.1, NP_059144.1).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Chemiluminescence

((0.03ug/ml) staining of Mouse Testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Immunohistochemistry (IHC)

((1ug/ml) staining of paraffin embedded Mouse Testis. Data kindly provided by Dr. Erwin Goldberg, Northwestern University, Evanston, IL USA.)

Related Product Information for anti-LDHC antibody

Lactate dehydrogenase (LDH) is an ubiquitous enzyme commonly found in wide variety of organisms, including plants and microbes. LDH is involved in the interconversion of the pyruvate and NADH to lactate and NAD+. It is also called Hydroxybutyrate Dehydrogenase (HBD), because it can catalyze the oxidation of hydroxybutyrate. In mammals, three types of LDH subunits (35kD) are encoded by the genes Ldh-A, Ldh-B, and Ldh-C. All LDH subunits can combine to form various terameric isoenzymes (140kD). LDHA and LDHB are expressed in most somatic tissues, while expression of LDHC is confined to the germinal epithelium of the testes.

Product Categories/Family for anti-LDHC antibody

Antibodies; Abs to Enzymes; Dehydrogenase

NCBI and Uniprot Product Information

• NCBI GI #: 9257228
• NCBI GeneID: 3948
• NCBI Accession #: NP_059144.1
• NCBI GenBank Nucleotide #: NM_017448.3
• UniProt Accession #: P07864; Q6GSG8; Q7Z7J4; D3DQY4
• Molecular Weight: 36,311 Da
• NCBI Official Full Name: L-lactate dehydrogenase C chain
• NCBI Official Synonym Full Names: lactate dehydrogenase C
• NCBI Official Symbol: LDHC
• NCBI Official Synonym Symbols: CT32; LDH3; LDHX
• NCBI Protein Information: L-lactate dehydrogenase C chain; LDH-C; LDH-X; LDH testis subunit; cancer/testis antigen 32; lactate dehydrogenase C4; lactate dehydrogenase c variant 1; lactate dehydrogenase c variant 3; lactate dehydrogenase c variant 4
• UniProt Protein Name: L-lactate dehydrogenase C chain
• Protein Family: L-lactate dehydrogenase
• UniProt Gene Name: LDHC
• UniProt Synonym Gene Names: LDH3; LDHX; LDH-C; CT32
• UniProt Entry Name: LDHC_HUMAN

NCBI Description

Lactate dehydrogenase C catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. LDHC is testis-specific and belongs to the lactate dehydrogenase family. Two transcript variants have been detected which differ in the 5' untranslated region. [provided by RefSeq, Jul 2008]

Uniprot Description

LDH-C: Possible role in sperm motility. Belongs to the LDH/MDH superfamily. LDH family.

Protein type: Oxidoreductase; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Carbohydrate Metabolism - pyruvate; Carbohydrate Metabolism - propanoate; Cancer Testis Antigen (CTA); EC 1.1.1.27; Amino Acid Metabolism - cysteine and methionine

Chromosomal Location of Human Ortholog: 11p15.1

Cellular Component: cytoplasm; nucleus

Molecular Function: L-lactate dehydrogenase activity

Biological Process: sperm motility; ATP biosynthetic process; cellular carbohydrate metabolic process; lactate oxidation; lactate biosynthetic process from pyruvate