Rabbit anti-Human LCP2 Polyclonal Antibody | anti-LCP2 antibody

LCP2 Antibody (N-Term)

Cat. Num. AAA9217348

• Gene Names: LCP2; SLP76; SLP-76
• Reactivity: Human
• Applications: Western Blot, ELISA
• Synonyms: LCP2; Polyclonal Antibody; LCP2 Antibody (N-Term); Lymphocyte cytosolic protein 2; SH2 domain-containing leukocyte protein of 76 kDa; SLP-76 tyrosine phosphoprotein; SLP76; Purified Rabbit Polyclonal Antibody (Pab); anti-LCP2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Ig
• Specificity: This LCP2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 46-80 amino acids from human LCP2.
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: 0.74 (varies by lot)
• Sequence Length: 533

• Applicable Applications for anti-LCP2 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB~~1:2000
• Cellular Location: Cytoplasm.
• Tissue Location: Highly expressed in spleen, thymus and peripheral blood leukocytes. Highly expressed also in T-cell and monocytic cell lines, expressed at lower level in B-cell lines Not detected in fibroblast or neuroblastoma cell lines
• Antigen Type: Synthesized Peptide
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Anti-LCP2 Antibody (N-Term) at 1:2000 dilution + Jurkat whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 60 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-LCP2 antibody

Involved in T-cell antigen receptor mediated signaling.

Product Categories/Family for anti-LCP2 antibody

Signal Transduction

References

Jackman J.K.,et al.J. Biol. Chem. 270:7029-7032(1995). Kalnine N.,et al.Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases. Ota T.,et al.Nat. Genet. 36:40-45(2004). Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases. Moog-Lutz C.,et al.J. Biol. Chem. 276:22375-22381(2001).

NCBI and Uniprot Product Information

• NCBI GI #: 10720065
• NCBI GeneID: 3937
• UniProt Accession #: Q13094; Q53XV4; A8KA25
• Molecular Weight: 60188 Da
• NCBI Official Full Name: Lymphocyte cytosolic protein 2
• NCBI Official Synonym Full Names: lymphocyte cytosolic protein 2
• NCBI Official Symbol: LCP2
• NCBI Official Synonym Symbols: SLP76; SLP-76
• NCBI Protein Information: lymphocyte cytosolic protein 2
• UniProt Protein Name: Lymphocyte cytosolic protein 2
• Protein Family: Larval cuticle protein
• UniProt Gene Name: LCP2
• UniProt Synonym Gene Names: SLP76
• UniProt Entry Name: LCP2_HUMAN

NCBI Description

SLP-76 was originally identified as a substrate of the ZAP-70 protein tyrosine kinase following T cell receptor (TCR) ligation in the leukemic T cell line Jurkat. The SLP-76 locus has been localized to human chromosome 5q33 and the gene structure has been partially characterized in mice. The human and murine cDNAs both encode 533 amino acid proteins that are 72% identical and comprised of three modular domains. The NH2-terminus contains an acidic region that includes a PEST domain and several tyrosine residues which are phosphorylated following TCR ligation. SLP-76 also contains a central proline-rich domain and a COOH-terminal SH2 domain. A number of additional proteins have been identified that associate with SLP-76 both constitutively and inducibly following receptor ligation, supporting the notion that SLP-76 functions as an adaptor or scaffold protein. Studies using SLP-76 deficient T cell lines or mice have provided strong evidence that SLP-76 plays a positive role in promoting T cell development and activation as well as mast cell and platelet function. [provided by RefSeq, Jul 2008]

Uniprot Description

SLP76: an adaptor protein involved in signaling through the T cell antigen receptor complex and signaling in other hematopoietic lineages including platelets. Originally identified as a substrate of the ZAP-70 protein tyrosine kinase following T cell receptor (TCR) ligation. Platelets deficient in SLP76 do not couple GPVI engagement with the signaling cascade that induces degranulation and aggregation in response to collagen. Contains four domains: a COOH-terminal SH2 domain, a central proline-rich domain, an NH2-terminal sterile {alpha} motif domain, and acidic region that contains three tyrosine phosphorylation motifs: Y112ESP, Y128ESP, and Y145EPP. pY112 and pY128 bind SH2 domains of Vav and Nck. pY145 is recognized by SH2 domains of the Tec family kinases Itk and Btk. Highly expressed in spleen, thymus, peripheral blood leukocytes, mast cells, platelets, and T-cell and monocytic cell lines. Expressed at lower level in B-cell lines. Not detected in fibroblast or neuroblasatoma cell lines.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 5q35.1

Cellular Component: cytosol

Molecular Function: protein binding

Biological Process: immune response; platelet activation; positive regulation of protein kinase activity; T cell receptor signaling pathway; transmembrane receptor protein tyrosine kinase signaling pathway