Western Blot (WB)
((0.5ug/ml) staining of Daudi (A) and U937 (B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat LCP1/plastin-2 Polyclonal Antibody | anti-LCP1 antibody
Goat anti-LCP1/plastin-2 (aa277-291) Antibody
Western Blot: Approx 70kDa band observed in lysates of cell lines Daudi and U937 (calculated MW of 70.3kDa according to NP_002289.2). Recommended concentration: 0.5-3ug/ml.
Western Blot (WB)
((0.5ug/ml) staining of Daudi (A) and U937 (B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.5ug/ml) staining of Daudi (A) and U937 (B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI Description
Plastins are a family of actin-binding proteins that are conserved throughout eukaryote evolution and expressed in most tissues of higher eukaryotes. In humans, two ubiquitous plastin isoforms (L and T) have been identified. Plastin 1 (otherwise known as Fimbrin) is a third distinct plastin isoform which is specifically expressed at high levels in the small intestine. The L isoform is expressed only in hemopoietic cell lineages, while the T isoform has been found in all other normal cells of solid tissues that have replicative potential (fibroblasts, endothelial cells, epithelial cells, melanocytes, etc.). However, L-plastin has been found in many types of malignant human cells of non-hemopoietic origin suggesting that its expression is induced accompanying tumorigenesis in solid tissues. [provided by RefSeq, Jul 2008]
Uniprot Description
L-plastin: Actin-binding protein. Plays a role in the activation of T-cells in response to costimulation through TCR/CD3 and CD2 or CD28. Modulates the cell surface expression of IL2RA/CD25 and CD69. Monomer. Interacts with AIF1. Detected in intestinal microvilli, hair cell stereocilia, and fibroblast filopodia, in spleen and other lymph node-containing organs. Expressed in peripheral blood T- lymphocytes, neutrophils, monocytes, B-lymphocytes, and myeloid cells.
Protein type: Motility/polarity/chemotaxis; Actin-binding
Chromosomal Location of Human Ortholog: 13q14.3
Cellular Component: actin cytoskeleton; actin filament; actin filament bundle; cytoplasm; cytosol; extracellular space; filopodium; focal adhesion; phagocytic cup; plasma membrane; podosome; ruffle; stress fiber
Molecular Function: actin binding; actin filament binding; calcium ion binding; GTPase binding; identical protein binding
Biological Process: actin filament bundle formation; cell migration; extracellular matrix disassembly; organ regeneration; regulation of intracellular protein transport; T cell activation during immune response
Research Articles on LCP1
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Product Notes
The LCP1 lcp1 (Catalog #AAA423297) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-LCP1/plastin-2 (aa277-291) Antibody reacts with Tested: Human; Expected from sequence similarity: Human and may cross-react with other species as described in the data sheet. AAA Biotech's LCP1/plastin-2 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 16000. Western Blot: Approx 70kDa band observed in lysates of cell lines Daudi and U937 (calculated MW of 70.3kDa according to NP_002289.2). Recommended concentration: 0.5-3ug/ml. Researchers should empirically determine the suitability of the LCP1 lcp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: HLENAGCNKI GNFST. It is sometimes possible for the material contained within the vial of "LCP1/plastin-2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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