Rabbit anti-Rat Lcn2 Polyclonal Antibody | anti-Lcn2 antibody

Lcn2 Antibody

Cat. Num. AAA7136120

• Gene Names: Lcn2; Sip24
• Reactivity: Rat
• Applications: ELISA, Immunohistochemistry
• Purity: Antigen Affinity Purified
• Synonyms: Lcn2; Polyclonal Antibody; Lcn2 Antibody; Neutrophil gelatinase-associated lipocalin (NGAL) (Alpha-2-microglobulin-related protein) (Alpha-2U globulin-related protein) (Lipocalin-2) (Siderocalin LCN2) (p25); anti-Lcn2 antibody

• Host: Rabbit
• Reactivity: Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Antigen Affinity Purified
• Form/Format: Liquid; Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, PH7.4

• Applicable Applications for anti-Lcn2 antibody: ELISA (EIA), Immunohistochemistry (IHC)
• Application Notes: IHC: 1:100-1:500
• Conjugation: Non-conjugated
• Immunogen: Recombinant Rat Lcn2 protein (1-198AA)
• Preparation and Storage: Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.

Immunohistochemistry (IHC)

(IHC image diluted at 1:200 and staining in paraffin-embedded rat lung tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.)

Related Product Information for anti-Lcn2 antibody

Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development (By similarity). Binds iron through association with 2,5-dihydroxybenzoic acid (2,5-DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis (By similarity). Involved in innate immunity; limits bacterial proliferation by sequestering iron bound to microbial siderophores, such as enterobactin. Can also bind siderophores from M.tuberculosis (By similarity).

Product Categories/Family for anti-Lcn2 antibody

Cardiovascular

NCBI and Uniprot Product Information

• NCBI GI #: 18543345
• NCBI GeneID: 170496
• NCBI Accession #: NP_570097.1
• NCBI GenBank Nucleotide #: NM_130741.1
• UniProt Accession #: P30152; Q5HZF1
• Molecular Weight: 22,476 Da
• NCBI Official Full Name: neutrophil gelatinase-associated lipocalin
• NCBI Official Synonym Full Names: lipocalin 2
• NCBI Official Symbol: Lcn2
• NCBI Official Synonym Symbols: Sip24
• NCBI Protein Information: neutrophil gelatinase-associated lipocalin; p25; NGAL; lipocalin-2; siderocalin LCN2; lipocalin 2 (oncogene 24p3); alpha-2U globulin-related protein; alpha-2-microglobulin-related protein
• UniProt Protein Name: Neutrophil gelatinase-associated lipocalin
• Protein Family: Neutrophil gelatinase-associated lipocalin
• UniProt Gene Name: Lcn2
• UniProt Synonym Gene Names: NGAL
• UniProt Entry Name: NGAL_RAT

NCBI Description

mouse homolog plays a role in IL3 withdrawal-induced apoptosis [RGD, Feb 2006]

Uniprot Description

LCN2: Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,5-dihydroxybenzoic acid (2,5- DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity, possibly by sequestrating iron, leading to limit bacterial growth. Belongs to the calycin superfamily. Lipocalin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transporter; Apoptosis; Secreted, signal peptide; Secreted

Cellular Component: extracellular space; cell; extracellular region; cytosol

Molecular Function: protein homodimerization activity; protease binding; transporter activity; iron ion binding

Biological Process: response to nutrient levels; response to drug; response to mycotoxin; response to virus; positive regulation of cell projection organization and biogenesis; response to herbicide; cellular response to nutrient levels; iron ion homeostasis; response to bacterium; siderophore transport; innate immune response; ion transport; response to oxidative stress