Western Blot (WB)
(Western blot analysis of extracts from Jurkat cells untreated, treated with UV or treated with H2O2 using Rabbit anti LCK (pTyr416)/FYN (pTyr416) antibody.H2O2 Treatment: Cells were cultured in serum free media for 16-20 hours before treatment with H2O2 (2mM) for 15 minutes UV Treatment: Cells were treated with UV for 15-30 minutes)
Rabbit anti-Human Lck/FYN Polyclonal Antibody | anti-LCK antibody
Rabbit anti Lck (pTyr416)/FYN (pTyr416)
Storage in frost-free freezers is not recommended. This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 12 months from date of despatch
Western Blot (WB)
(Western blot analysis of extracts from Jurkat cells untreated, treated with UV or treated with H2O2 using Rabbit anti LCK (pTyr416)/FYN (pTyr416) antibody.H2O2 Treatment: Cells were cultured in serum free media for 16-20 hours before treatment with H2O2 (2mM) for 15 minutes UV Treatment: Cells were treated with UV for 15-30 minutes)
Western Blot (WB)
(Western blot analysis of extracts from Jurkat cells untreated, treated with UV or treated with H2O2 using Rabbit anti LCK (pTyr416)/FYN (pTyr416) antibody.H2O2 Treatment: Cells were cultured in serum free media for 16-20 hours before treatment with H2O2 (2mM) for 15 minutes UV Treatment: Cells were treated with UV for 15-30 minutes)
LCK and FYN are members of the Src family of tyrosine kinases that are involved in the regulation of numerous processes including differentiation and proliferation. Autophosphorylation of LCK and FYN at tyrosine 416 increases kinase activity via an open confirmation of the LCK and FYN which makes substrates more accessible (Boggon and Eck 2004, Irtegun et al. 2004).
NCBI and Uniprot Product Information
NCBI Description
This gene is a member of the protein-tyrosine kinase oncogene family. It encodes a membrane-associated tyrosine kinase that has been implicated in the control of cell growth. The protein associates with the p85 subunit of phosphatidylinositol 3-kinase and interacts with the fyn-binding protein. Alternatively spliced transcript variants encoding distinct isoforms exist. [provided by RefSeq, Jul 2008]
Uniprot Description
Non-receptor tyrosine-protein kinase that plays a role in many biological processes including regulation of cell growth and survival, cell adhesion, integrin-mediated signaling, cytoskeletal remodeling, cell motility, immune response and axon guidance. Inactive FYN is phosphorylated on its C-terminal tail within the catalytic domain. Following activation by PKA, the protein subsequently associates with PTK2/FAK1, allowing PTK2/FAK1 phosphorylation, activation and targeting to focal adhesions. Involved in the regulation of cell adhesion and motility through phosphorylation of CTNNB1 (beta-catenin) and CTNND1 (delta-catenin). Regulates cytoskeletal remodeling by phosphorylating several proteins including the actin regulator WAS and the microtubule-associated proteins MAP2 and MAPT. Promotes cell survival by phosphorylating AGAP2/PIKE-A and preventing its apoptotic cleavage. Participates in signal transduction pathways that regulate the integrity of the glomerular slit diaphragm (an essential part of the glomerular filter of the kidney) by phosphorylating several slit diaphragm components including NPHS1, KIRREL1 and TRPC6. Plays a role in neural processes by phosphorylating DPYSL2, a multifunctional adapter protein within the central nervous system, ARHGAP32, a regulator for Rho family GTPases implicated in various neural functions, and SNCA, a small pre-synaptic protein. Participates in the downstream signaling pathways that lead to T-cell differentiation and proliferation following T-cell receptor (TCR) stimulation. Also participates in negative feedback regulation of TCR signaling through phosphorylation of PAG1, thereby promoting interaction between PAG1 and CSK and recruitment of CSK to lipid rafts. CSK maintains LCK and FYN in an inactive form. Promotes CD28-induced phosphorylation of VAV1.
Research Articles on LCK
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Product Notes
The LCK fyn (Catalog #AAA225277) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti Lck (pTyr416)/FYN (pTyr416) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Lck/FYN can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:2000. Researchers should empirically determine the suitability of the LCK fyn for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Lck/FYN, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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