Rabbit anti-Human, Mouse LATS2 Polyclonal Antibody | anti-LATS2 antibody

LATS2 Rabbit pAb

Cat. Num. AAA4754725

• Gene Names: LATS2; KPM
• Reactivity: Human, Mouse
• Applications: ELISA, Immunohistochemistry
• Purity: Affinity purified
• Synonyms: LATS2; Polyclonal Antibody; LATS2 Rabbit pAb; large tumor suppressor kinase 2; anti-LATS2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Expressed at high levels in heart and skeletal muscle and at lower levels in all other tissues examined.
• Purity/Purification: Affinity purified
• Form/Format: pH7.4 PBS, 0.05% NaN3, 40% Glycerol

• Applicable Applications for anti-LATS2 antibody: ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: ELISA: 1:1000-1:2000; IHC: 1:25-1:100
• Immunogen: Synthetic peptide of human LATS2
• Conjugation: Unconjugated
• Molecular Function: Negative regulator of YAP1 in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Acts as a tumor suppressor which plays a critical role in centrosome duplication, maintenance of mitotic fidelity and genomic stability. Negatively regulates G1/S transition by down-regulating cyclin E/CDK2 kinase activity. Negative regulator of the androgen receptor. Phosphorylates SNAI1 in the nucleus leading to its nuclear retention and stabilization, which enhances its epithelial-mesenchymal transition and tumor cell invasion/migration activities. This tumor-promoting activity is independent of its effects upon YAP1 or WWTR1/TAZ.
• Modification: Unmodified
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Immunohistochemistry (IHC)

(The image is immunohistochemistry of paraffin-embedded Human liver cancer tissue using 160914(LATS2 Antibody) at dilution 1/30.(Original magnification: ×200))

Immunohistochemistry (IHC)

(The image is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using 160914(LATS2 Antibody) at dilution 1/30. (Original magnification: ×200))

Related Product Information for anti-LATS2 antibody

This gene encodes a serine/threonine protein kinase belonging to the LATS tumor suppressor family. The protein localizes to centrosomes during interphase, and early and late metaphase. It interacts with the centrosomal proteins aurora-A and ajuba and is required for accumulation of gamma-tubulin and spindle formation at the onset of mitosis. It also interacts with a negative regulator of p53 and may function in a positive feedback loop with p53 that responds to cytoskeleton damage. Additionally, it can function as a co-repressor of androgen-responsive gene expression.

Product Categories/Family for anti-LATS2 antibody

Primary antibody

NCBI and Uniprot Product Information

• NCBI GI #: 126507090
• NCBI GeneID: 26524
• NCBI Accession #: NM_014572
• NCBI GenBank Nucleotide #: NM_014572
• UniProt Accession #: Q9NRM7; Q9P2X1; B1AM47
• Molecular Weight: ~98 kDa
• NCBI Official Full Name: Homo sapiens large tumor suppressor kinase 2 (LATS2), mRNA
• NCBI Official Synonym Full Names: large tumor suppressor kinase 2
• NCBI Official Symbol: LATS2
• NCBI Official Synonym Symbols: KPM
• NCBI Protein Information: serine/threonine-protein kinase LATS2; warts-like kinase; serine/threonine kinase KPM; large tumor suppressor homolog 2; serine/threonine-protein kinase kpm; LATS, large tumor suppressor, homolog 2; kinase phosphorylated during mitosis protein; LATS (larg
• UniProt Protein Name: Serine/threonine-protein kinase LATS2
• Protein Family: Serine/threonine-protein kinase
• UniProt Gene Name: LATS2
• UniProt Synonym Gene Names: KPM
• UniProt Entry Name: LATS2_HUMAN

NCBI Description

This gene encodes a serine/threonine protein kinase belonging to the LATS tumor suppressor family. The protein localizes to centrosomes during interphase, and early and late metaphase. It interacts with the centrosomal proteins aurora-A and ajuba and is required for accumulation of gamma-tubulin and spindle formation at the onset of mitosis. It also interacts with a negative regulator of p53 and may function in a positive feedback loop with p53 that responds to cytoskeleton damage. Additionally, it can function as a co-repressor of androgen-responsive gene expression. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Negative regulator of YAP1 in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Acts as a tumor suppressor which plays a critical role in centrosome duplication, maintenance of mitotic fidelity and genomic stability. Negatively regulates G1/S transition by down-regulating cyclin E/CDK2 kinase activity. Negative regulator of the androgen receptor. Phosphorylates SNAI1 in the nucleus leading to its nuclear retention and stabilization, which enhances its epithelial-mesenchymal transition and tumor cell invasion/migration activities. This tumor-promoting activity is independent of its effects upon YAP1 or WWTR1/TAZ. Ref.1 Ref.6 Ref.8 Ref.11 Ref.15

Catalytic activity: ATP + a protein = ADP + a phosphoprotein.

Cofactor: Magnesium.

Subunit structure: Interacts with and is phosphorylated by AURKA. Binds to AR. Interacts with AJUBA during mitosis and this complex regulates organization of the spindle apparatus through recruitment of gamma-tubulin to the centrosome. Interacts (via PPxY motif) with YAP1 (via WW domains). Interacts with MOB1A and MOB1B. Interacts with LIMD1, WTIP and AJUBA. Interacts with SNAI1. Ref.7 Ref.8 Ref.10 Ref.11 Ref.13 Ref.14 Ref.15

Subcellular location: Cytoplasm › cytoskeleton › microtubule organizing center › centrosome. Cytoplasm. Cytoplasm › cytoskeleton › spindle pole. Nucleus. Note: Colocalizes with AURKA at the centrosomes during interphase, early prophase and cytokinesis. Migrates to the spindle poles during mitosis, and to the midbody during cytokinesis. Translocates to the nucleus upon mitotic stress by nocodazole treatment. Ref.6 Ref.7 Ref.15

Tissue specificity: Expressed at high levels in heart and skeletal muscle and at lower levels in all other tissues examined. Ref.1 Ref.5

Post-translational modification: Autophosphorylated and phosphorylated during M-phase and the G1/S-phase of the cell cycle. Phosphorylated and activated by STK3/MST2. Ref.1 Ref.7 Ref.9 Ref.15

Sequence similarities: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.Contains 1 AGC-kinase C-terminal domain.Contains 1 protein kinase domain.Contains 1 UBA domain.

Sequence caution: The sequence BAA92381.1 differs from that shown. Reason: Frameshift at positions 512 and 526.