Rabbit anti-Human Latexin Polyclonal Antibody | anti-LXN antibody

Latexin, ID (LXN, Latexin, Endogenous carboxypeptidase inhibitor, Protein MUM, Tissue carboxypeptidase inhibitor) (Biotin)

Cat. Num. AAA6315296

• Gene Names: LXN; ECI; TCI
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, ELISA, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: Latexin; Polyclonal Antibody; ID (LXN; Endogenous carboxypeptidase inhibitor; Protein MUM; Tissue carboxypeptidase inhibitor) (Biotin); LXN; Tissue carboxypeptidase inhibitor; anti-LXN antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-LXN antibody: Flow Cytometry (FC/FACS), ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; ELISA: 1:1,000; IHC: 1:10-50; WB: 1:50-100; Applications are based on unconjugated antibody.
• Immunogen: Latexin antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 85~115 amino acids from the Center region of human LXN.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of Latexin (arrow) using rabbit polyclonal Latexin Antibody (Center) (RB12661). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the LXN gene (Lane 2) (Origene Technologies).)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with Latexin antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human Spleen tissue reacted with Latexin antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of WiDr cells using Latexin Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-LXN antibody

Latexin possesses nearly irreversible, non-competitive and potent inhibition of zinc-dependent metallocarboxypeptidases CPA1, CPA2, and CPA4. It is expressed in a neuronal subset in the cerebral cortex and cells in other neural and non-neural tissues of rat. Latexin plays a role in regional specification and/or morphogenesis of the forebrain.

Product Categories/Family for anti-LXN antibody

Antibodies; Enzymes; Carboxypeptidase

References

Pallares, I., Proc. Natl. Acad. Sci. U.S.A. 102 (11), 3978-3983 (2005).

NCBI and Uniprot Product Information

• NCBI GI #: 194473714
• NCBI GeneID: 56925
• NCBI Accession #: NP_064554.3
• NCBI GenBank Nucleotide #: NM_020169.3
• UniProt Accession #: Q9BS40; Q96PN2; Q9NQS6
• Molecular Weight: 25,750 Da
• NCBI Official Full Name: latexin
• NCBI Official Synonym Full Names: latexin
• NCBI Official Symbol: LXN
• NCBI Official Synonym Symbols: ECI; TCI
• NCBI Protein Information: latexin; MUM; endogenous carboxypeptidase inhibitor; tissue carboxypeptidase inhibitor
• UniProt Protein Name: Latexin
• Protein Family: Latexin
• UniProt Gene Name: LXN
• UniProt Synonym Gene Names: ECI; TCI
• UniProt Entry Name: LXN_HUMAN

NCBI Description

This gene encodes the only known protein inhibitor of zinc-dependent metallocarboxypeptidases. [provided by RefSeq, Oct 2008]

Uniprot Description

LXN: Hardly reversible, non-competitive, and potent inhibitor of CPA1, CPA2 and CPA4. Belongs to the protease inhibitor I47 (latexin) family.

Chromosomal Location of Human Ortholog: 3q25.32

Cellular Component: cytoplasm

Molecular Function: metalloendopeptidase inhibitor activity; heparin binding

Biological Process: detection of temperature stimulus involved in sensory perception of pain; inflammatory response