Sheep anti-Mouse Latexin Polyclonal Antibody | anti-LXN antibody
Latexin (LXN)
Gene Names
LXN; ECI; TCI
Reactivity
Mouse
Applications
ELISA, Western Blot, Immunoprecipitation, Neutralization Assay
Purity
Affinity PurifiedPurified by mouse Latexin affinity chromatography.
Synonyms
Latexin; Polyclonal Antibody; Latexin (LXN); Anti -Latexin (LXN); anti-LXN antibody
Host
Sheep
Reactivity
Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Selected for its ability to recognize mouse Latexin in direct ELISAs and Western Blots. In these formats, this antibody shows ~40% crossreactivity with rhLatexin.
Purity/Purification
Affinity Purified
Purified by mouse Latexin affinity chromatography.
Purified by mouse Latexin affinity chromatography.
Form/Format
Supplied as a lyophilized powder from a 0.2um filtered solution in PBS with 5% trehalose. Reconstitute with sterile PBS, 40-50% glycerol. If 0.5ml is used, the concentration will be 0.2mg/ml.
Applicable Applications for anti-LXN antibody
ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Neutralization
Application Notes
Western Blot: This antibody can be used at 0.1-0.2ug/ml with the appropriate secondary reagents to detect mouse Latexin. The detection limit for rmLatexin is approximately 5 ng/lane and 0.5 ng/lane under non-reducing and reducing conditions, respectively., Immunoprecipitation: This antibody has been used to immunoprecipitate rmLatexin from conditioned media of transfected NS0 cells., Neutralization: This antibody has been used to neutralize the inhibitory activity of rmLatexin against rmCPA. The antibody was preincubated with the inhibitor at different molar ratios at room temperature for 30 minutes. The resulting mixtures were then incubated with the enzyme at room temperature for 60 minutes. The enzyme was assayed with a peptide substrate. 50% of the proteolytic activity was restored by the antibody at 4.4ug/ml (IC50) under conditions in which 0.1ug/ml of the inhibitor, 0.1ug/ml of the enzyme, and 100 M of the substrate were present. Considering the molecular masses of the inhibitor (26kD) and the antibody (150kD), IC50 was achieved at approximately 8:1 molar ratio of the antibody to the inhibitor., Direct ELISA: This antibody can be used at 0.5-1.0ug/ml with the appropriate secondary reagents to detect mouse Latexin. The detection limit for rmLatexin is approximately 0.3 ng/well., Optimal dilutions should be determined by each laboratory for each application.
Immunogen
E. coli-derived rmLatexin (aa2-222)
Preparation and Storage
Lyophilized powder may be stored at 4 degree C for short-term only. Reconstitute to nominal volume by adding sterile PBS with 40-50% glycerol and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Product Categories/Family for anti-LXN antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
25,750 Da
NCBI Official Full Name
latexin
NCBI Official Synonym Full Names
latexin
NCBI Official Symbol
LXN
NCBI Official Synonym Symbols
ECI; TCI
NCBI Protein Information
latexin; MUM; OTTHUMP00000213517; tissue carboxypeptidase inhibitor; endogenous carboxypeptidase inhibitor
UniProt Protein Name
Latexin
Protein Family
UniProt Gene Name
LXN
UniProt Entry Name
LXN_HUMAN
NCBI Description
This gene encodes the only known protein inhibitor of zinc-dependent metallocarboxypeptidases. [provided by RefSeq]
Uniprot Description
LXN: Hardly reversible, non-competitive, and potent inhibitor of CPA1, CPA2 and CPA4. Belongs to the protease inhibitor I47 (latexin) family.
Chromosomal Location of Human Ortholog: 3q25.32
Cellular Component: cytoplasm
Molecular Function: heparin binding; metalloendopeptidase inhibitor activity
Biological Process: detection of temperature stimulus involved in sensory perception of pain; inflammatory response
Similar Products
Product Notes
The LXN lxn (Catalog #AAA618718) is an Antibody produced from Sheep and is intended for research purposes only. The product is available for immediate purchase. The Latexin (LXN) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Latexin can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Neutralization. Western Blot: This antibody can be used at 0.1-0.2ug/ml with the appropriate secondary reagents to detect mouse Latexin. The detection limit for rmLatexin is approximately 5 ng/lane and 0.5 ng/lane under non-reducing and reducing conditions, respectively., Immunoprecipitation: This antibody has been used to immunoprecipitate rmLatexin from conditioned media of transfected NS0 cells., Neutralization: This antibody has been used to neutralize the inhibitory activity of rmLatexin against rmCPA. The antibody was preincubated with the inhibitor at different molar ratios at room temperature for 30 minutes. The resulting mixtures were then incubated with the enzyme at room temperature for 60 minutes. The enzyme was assayed with a peptide substrate. 50% of the proteolytic activity was restored by the antibody at 4.4ug/ml (IC50) under conditions in which 0.1ug/ml of the inhibitor, 0.1ug/ml of the enzyme, and 100 M of the substrate were present. Considering the molecular masses of the inhibitor (26kD) and the antibody (150kD), IC50 was achieved at approximately 8:1 molar ratio of the antibody to the inhibitor., Direct ELISA: This antibody can be used at 0.5-1.0ug/ml with the appropriate secondary reagents to detect mouse Latexin. The detection limit for rmLatexin is approximately 0.3 ng/well., Optimal dilutions should be determined by each laboratory for each application. Researchers should empirically determine the suitability of the LXN lxn for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Latexin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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