Western Blot (WB)
(Anti-LASP1 antibody, MBS175220, Western blottingLane 1: Rat Liver Tissue LysateLane 2: Rat Spleen Tissue LysateLane 3: Rat Intestine Tissue LysateLane 4: JURKAT Cell LysateLane 5: MCF-7 Cell LysateLane 6: A431 Cell LysateLane 7: HELA Cell LysateLane 8: 293T Cell Lysate )
Rabbit LASP1 Polyclonal Antibody | anti-LASP1 antibody
Anti-LASP1 antibody
Gene Names
LASP1; MLN50; Lasp-1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry
Purity
Immunogen affinity purified.
Synonyms
LASP1; Polyclonal Antibody; Anti-LASP1 antibody; LIM and SH3 domain protein 1(LASP-1); LIM and SH3 protein 1; LASP 1 antibody; LASP-1 antibody; LASP1 antibody; LASP1_HUMAN antibody; LIM and SH3 domain protein 1 antibody; LIM and SH3 protein 1 antibody; Metastatic lymph node gene 50 protein antibody; MLN 50 antibody; MLN50 antibody; OTTHUMP00000164238 antibody; OTTHUMP00000164239 antibody; anti-LASP1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
No cross ractivity with other proteins.
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Sequence Length
261
Applicable Applications for anti-LASP1 antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC)
Application Notes
Western Blot (WB): Concentration: 0.1-0.5ug/ml; Tested Species: Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section): 0.5-1ug/ml; Tested Species: Human; Predicted Species: Mouse, Rat; Antigen Retrieval: By Heat
Immunocytochemistry (ICC): Concentration: 0.5-1ug/ml; Tested Species: Human; Predicted Species: Mouse, Rat
Tested Species: In-house tested species with positive results.
Predicted Species: Species predicted to be fit for the product based on sequence similarities.
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20mins is required for the staining of formalin/paraffin sections..
Other applications have not been tested.
Optimal dilutions should be determined by end user.
Immunohistochemistry (Paraffin-embedded Section): 0.5-1ug/ml; Tested Species: Human; Predicted Species: Mouse, Rat; Antigen Retrieval: By Heat
Immunocytochemistry (ICC): Concentration: 0.5-1ug/ml; Tested Species: Human; Predicted Species: Mouse, Rat
Tested Species: In-house tested species with positive results.
Predicted Species: Species predicted to be fit for the product based on sequence similarities.
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20mins is required for the staining of formalin/paraffin sections..
Other applications have not been tested.
Optimal dilutions should be determined by end user.
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human LASP1 (16-30aa EKVNCLDKFWHKACF), identical to the related rat sequence and different from the related mouse sequence by one amino acid.
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Western Blot (WB)
(Anti-LASP1 antibody, MBS175220, Western blottingLane 1: Rat Liver Tissue LysateLane 2: Rat Spleen Tissue LysateLane 3: Rat Intestine Tissue LysateLane 4: JURKAT Cell LysateLane 5: MCF-7 Cell LysateLane 6: A431 Cell LysateLane 7: HELA Cell LysateLane 8: 293T Cell Lysate )
Western Blot (WB)
(Anti-LASP1 antibody, MBS175220, Western blottingLane 1: Rat Liver Tissue LysateLane 2: Rat Spleen Tissue LysateLane 3: Rat Intestine Tissue LysateLane 4: JURKAT Cell LysateLane 5: MCF-7 Cell LysateLane 6: A431 Cell LysateLane 7: HELA Cell LysateLane 8: 293T Cell Lysate )
Immunohistochemistry (IHC)
(Anti-LASP1 antibody, MBS175220, IHC(P)IHC(P): Human Lung Cancer Tissue )
Immunohistochemistry (IHC)
(Anti-LASP1 antibody, MBS175220, IHC(P)IHC(P): Human Lung Cancer Tissue )
Immunocytochemistry (ICC)
(Anti-LASP1 antibody, MBS175220, ICCICC: A549 Cell )
Immunocytochemistry (ICC)
(Anti-LASP1 antibody, MBS175220, ICCICC: A549 Cell )
Related Product Information for anti-LASP1 antibody
Description: Rabbit IgG polyclonal antibody for LIM and SH3 domain protein 1(LASP1) detection. Tested with WB, IHC-P, ICC in Human, Mouse, Rat.
Background: LASP1(LIM and SH3 domain protein 1), also known as LASP1, is a protein that in humans is encoded by the LASP1 gene. This gene encodes a member of a LIM protein subfamily which is characterized by a LIM motif and a domain of Src homology region 3. This protein functions as an actin-binding protein and possibly in cytoskeletal organization. LASP1 has been shown to interact with Zyxin. Tomasetto et al.(1995) mapped the LASP1 gene to chromosome 17q11-q21.3 by radioactive in situ hybridization. Northern blot analysis revealed that LASP1 mRNA was expressed at a basal level in all normal tissues examined and overexpressed in 8% of primary breast cancers. In most of these cancers, LASP1 and ERBB2 were simultaneously overexpressed.
Background: LASP1(LIM and SH3 domain protein 1), also known as LASP1, is a protein that in humans is encoded by the LASP1 gene. This gene encodes a member of a LIM protein subfamily which is characterized by a LIM motif and a domain of Src homology region 3. This protein functions as an actin-binding protein and possibly in cytoskeletal organization. LASP1 has been shown to interact with Zyxin. Tomasetto et al.(1995) mapped the LASP1 gene to chromosome 17q11-q21.3 by radioactive in situ hybridization. Northern blot analysis revealed that LASP1 mRNA was expressed at a basal level in all normal tissues examined and overexpressed in 8% of primary breast cancers. In most of these cancers, LASP1 and ERBB2 were simultaneously overexpressed.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
LIM and SH3 domain protein 1
NCBI Official Synonym Full Names
LIM and SH3 protein 1
NCBI Official Symbol
LASP1
NCBI Official Synonym Symbols
MLN50; Lasp-1
NCBI Protein Information
LIM and SH3 domain protein 1; metastatic lymph node gene 50 protein
UniProt Protein Name
LIM and SH3 domain protein 1
Protein Family
UniProt Gene Name
LASP1
UniProt Synonym Gene Names
MLN50; LASP-1; MLN 50
UniProt Entry Name
LASP1_HUMAN
NCBI Description
This gene encodes a member of a subfamily of LIM proteins, characterized by a LIM motif and a domain of Src homology region 3, and also a member of the nebulin family of actin-binding proteins. The encoded protein is a cAMP and cGMP dependent signaling protein and binds to the actin cytoskeleton at extensions of the cell membrane. The encoded protein has been linked to metastatic breast cancer, hematopoetic tumors such as B-cell lymphomas, and colorectal cancer. [provided by RefSeq, Oct 2012]
Uniprot Description
Lasp-1: a member of the LIM protein subfamily, characterized by a LIM motif and SH3 domain. Functions as an actin-binding protein, possibly in cytoskeletal organization.
Protein type: Actin-binding; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 17q11-q21.3
Cellular Component: cortical actin cytoskeleton; focal adhesion
Molecular Function: actin filament binding; protein binding; SH3/SH2 adaptor activity; zinc ion binding; ion transmembrane transporter activity
Biological Process: positive regulation of signal transduction; ion transport
Research Articles on LASP1
Similar Products
Product Notes
The LASP1 lasp1 (Catalog #AAA175220) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-LASP1 antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's LASP1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC). Western Blot (WB): Concentration: 0.1-0.5ug/ml; Tested Species: Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section): 0.5-1ug/ml; Tested Species: Human; Predicted Species: Mouse, Rat; Antigen Retrieval: By Heat Immunocytochemistry (ICC): Concentration: 0.5-1ug/ml; Tested Species: Human; Predicted Species: Mouse, Rat Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end user. Researchers should empirically determine the suitability of the LASP1 lasp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LASP1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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