Rabbit anti-Human LASP1 Polyclonal Antibody | anti-LASP1 antibody

LASP1, ID (LASP1, MLN50, LIM and SH3 domain protein 1, Metastatic lymph node gene 50 protein) (AP)

Cat. Num. AAA6315261

• Gene Names: LASP1; MLN50; Lasp-1
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: LASP1; Polyclonal Antibody; ID (LASP1; MLN50; LIM and SH3 domain protein 1; Metastatic lymph node gene 50 protein) (AP); Metastatic lymph node gene 50 protein; anti-LASP1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-LASP1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: LASP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 119-148 amino acids from the Central region of human LASP1.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(LASP1 Antibody (Center) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the LASP1 antibody detected the LASP1 protein (arrow).)

Related Product Information for anti-LASP1 antibody

This gene encodes a member of a LIM protein subfamily characterized by a LIM motif and a domain of Src homology region 3. The encoded protein functions as an actin-binding protein and possibly in cytoskeletal organization.

Product Categories/Family for anti-LASP1 antibody

Antibodies; Actin Related Proteins

References

Traenka, C., et al. Cancer Res. 70(20):8003-8014(2010). Zhao, L., et al. FEBS J. 277(20):4195-4204(2010). Zhao, L., et al. Gut 59(9):1226-1235(2010). Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Frietsch, J.J., et al. Br. J. Cancer 102(11):1645-1653(2010).

NCBI and Uniprot Product Information

• NCBI GI #: 5453710
• NCBI GeneID: 3927
• NCBI Accession #: NP_006139.1
• NCBI GenBank Nucleotide #: NM_006148.3
• UniProt Accession #: Q14847; Q96ED2; Q96IG0; B4DGQ0
• Molecular Weight: 29,717 Da
• NCBI Official Full Name: LIM and SH3 domain protein 1 isoform a
• NCBI Official Synonym Full Names: LIM and SH3 protein 1
• NCBI Official Symbol: LASP1
• NCBI Official Synonym Symbols: MLN50; Lasp-1
• NCBI Protein Information: LIM and SH3 domain protein 1; metastatic lymph node gene 50 protein
• UniProt Protein Name: LIM and SH3 domain protein 1
• Protein Family: LIM and SH3 domain protein
• UniProt Gene Name: LASP1
• UniProt Synonym Gene Names: MLN50; LASP-1; MLN 50
• UniProt Entry Name: LASP1_HUMAN

NCBI Description

This gene encodes a member of a subfamily of LIM proteins, characterized by a LIM motif and a domain of Src homology region 3, and also a member of the nebulin family of actin-binding proteins. The encoded protein is a cAMP and cGMP dependent signaling protein and binds to the actin cytoskeleton at extensions of the cell membrane. The encoded protein has been linked to metastatic breast cancer, hematopoetic tumors such as B-cell lymphomas, and colorectal cancer. [provided by RefSeq, Oct 2012]

Uniprot Description

Lasp-1: a member of the LIM protein subfamily, characterized by a LIM motif and SH3 domain. Functions as an actin-binding protein, possibly in cytoskeletal organization.

Protein type: Actin-binding; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 17q11-q21.3

Cellular Component: cortical actin cytoskeleton; focal adhesion

Molecular Function: actin filament binding; protein binding; SH3/SH2 adaptor activity; zinc ion binding; ion transmembrane transporter activity

Biological Process: positive regulation of signal transduction; ion transport