Rabbit anti-Human LARP6 Polyclonal Antibody | anti-LARP6 antibody

LARP6, NT (LARP6, La-related protein 6, Acheron, La ribonucleoprotein domain family member 6) (AP)

Cat. Num. AAA6315206

• Gene Names: LARP6; ACHN
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: LARP6; Polyclonal Antibody; NT (LARP6; La-related protein 6; Acheron; La ribonucleoprotein domain family member 6) (AP); La ribonucleoprotein domain family member 6; anti-LARP6 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human LARP6.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-LARP6 antibody: Immunohistochemistry (IHC) Paraffin, ELISA (EIA), Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 7-35 in the N-terminal region of human LARP6.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in 293 cell line lysates (35ug/lane) usingMBS6007777.This demonstrates MBS6007777 detected the LARP6 protein (arrow).)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded mouse heart tissue using MBS647896 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS647896 for immunohistochemistry.)

Testing Data

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Product Categories/Family for anti-LARP6 antibody

Antibodies; Nuclear Proteins

References

1. Cai, L., et al. J. Mol. Biol. 395(2):309-326(2010). 2. Glenn, H.L., et al. Am. J. Physiol., Cell Physiol. 298 (1), C46-C55 (2010). 3. Valavanis, C., et al. Gene 393 (1-2), 101-109 (2007). 4. Olsen, J.V., et al. Cell 127(3):635-648(2006). 5. Olsen, J.V., et al. Cell 127(3):635-648(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 37537710
• NCBI GeneID: 55323
• NCBI Accession #: NP_060827.2; NP_932062.1
• NCBI GenBank Nucleotide #: NM_018357.3
• UniProt Accession #: Q9BRS8; Q5XKE4; Q8N3N2; Q9NUR0
• Molecular Weight: 54737
• NCBI Official Full Name: la-related protein 6 isoform 1
• NCBI Official Synonym Full Names: La ribonucleoprotein domain family, member 6
• NCBI Official Symbol: LARP6
• NCBI Official Synonym Symbols: ACHN
• NCBI Protein Information: la-related protein 6
• UniProt Protein Name: La-related protein 6
• Protein Family: La-related protein
• UniProt Gene Name: LARP6
• UniProt Synonym Gene Names: Achn
• UniProt Entry Name: LARP6_HUMAN

Uniprot Description

Function: Regulates the coordinated translation of type I collagen alpha-1 and alpha-2 mRNAs, CO1A1 and CO1A2. Stabilizes mRNAs through high-affinity binding of a stem-loop structure in their 5' UTR. This regulation requires VIM and MYH10 filaments, and the helicase DHX9. Ref.7 Ref.8 Ref.9

Subunit structure: Interacts (via the HTH domain) with VIM/vimentin. Interacts (via C-terminus) with non-muscle myosin MYH10. Interacts (via C-terminus) with DHX9. Ref.7 Ref.8 Ref.9

Subcellular location: Cytoplasm. Nucleus. Note: Shuttles between the nucleus and the cytoplasm. Ref.1

Tissue specificity: Expressed in numerous tissues. Ref.1

Domain: The RRM domain mediates the association with collagen mRNAs stem-loops.

Sequence similarities: Contains 1 HTH La-type RNA-binding domain.Contains 1 RRM (RNA recognition motif) domain.