Rabbit LAMP-1 Polyclonal Antibody | anti-LAMP1 antibody

LAMP-1 Antibody

Cat. Num. AAA151533

• Gene Names: LAMP1; LAMPA; CD107a; LGP120
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: LAMP-1 Antibody is affinity chromatography purified via peptide column.
• Synonyms: LAMP-1; Polyclonal Antibody; LAMP-1 Antibody; LAMPA; CD107a; LGP120; Lysosome-associated membrane glycoprotein 1; CD107 antigen-like family member A; lysosomal-associated membrane protein 1; anti-LAMP1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: LAMP-1 antibody is predicted to not cross-react with LAMP-2. Due to extensive post-translational modification, LAMP-1 often migrates at higher than predicted molecular weight in SDS-PAGE.
• Purity/Purification: LAMP-1 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 417

• Applicable Applications for anti-LAMP1 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: LAMP-1 antibody can be used for the detection of LAMP-1 by Western blot at 1 - 2 mug/mL. Antibody can also be used for immunohistochemistry starting at 20 mug/mL. For immunofluorescence start at 20 mug/mL.
• Conjugate: Unconjugated
• Immunogen: LAMP-1 antibody was raised against a 15 amino acid synthetic peptide from near the center of human LAMP-1.
• Buffer: LAMP-1 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: LAMP-1 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of LAMP-1 in EL4 cell lysate with LAMP-1 antibody at (A) 1 and (B) 2 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of LAMP-1 in human colon tissue with LAMP-1 antibody at 20 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of LAMP-1 in Human Colon cells with LAMP-1 antibody at 20 μg/mL.)

Immunohistochemistry (IHC)

(Immunohistochemistry of LAMP-1 in rat colon tissue with LAMP-1 antibody at 2.5 μg/ml.)

Immunofluorescence (IF)

(Immunofluorescence of LAMP-1 in rat colon tissue with LAMP-1 antibody at 20 μg/ml.)

Related Product Information for anti-LAMP1 antibody

LAMP-1 Antibody: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components and is negatively regulated by TOR (Target of rapamycin). A protein recently found to be involved in autophagy, LAMP-2, is a highly glycosylated protein associated with the lysosome. LAMP-1 shares much homology to LAMP-2 and is thought to have overlapping functions. Mice lacking LAMP-1 had very minor defects compared to those deficient in LAMP-2 expression. However, the loss of both proteins resulted in embryonic lethality, suggesting that each protein possesses some unique and necessary functions.

NCBI and Uniprot Product Information

• NCBI GI #: 112380628
• NCBI GeneID: 3916
• NCBI Accession #: NP_005552
• NCBI GenBank Nucleotide #: NM_005561.3
• UniProt Accession #: P11279; Q8WU33; Q96I40; Q9BRD2; Q9NP13; B4DWL3
• Molecular Weight: Predicted: 46 kDa; ; Observed: 100 kDa
• NCBI Official Full Name: lysosome-associated membrane glycoprotein 1
• NCBI Official Synonym Full Names: lysosomal-associated membrane protein 1
• NCBI Official Symbol: LAMP1
• NCBI Official Synonym Symbols: LAMPA; CD107a; LGP120
• NCBI Protein Information: lysosome-associated membrane glycoprotein 1; LAMP-1; CD107 antigen-like family member A; lysosome-associated membrane protein 1
• UniProt Protein Name: Lysosome-associated membrane glycoprotein 1
• Protein Family: Lysosome-associated membrane glycoprotein
• UniProt Gene Name: LAMP1
• UniProt Synonym Gene Names: LAMP-1; Lysosome-associated membrane protein 1
• UniProt Entry Name: LAMP1_HUMAN

NCBI Description

The protein encoded by this gene is a member of a family of membrane glycoproteins. This glycoprotein provides selectins with carbohydrate ligands. It may also play a role in tumor cell metastasis. [provided by RefSeq, Jul 2008]

Uniprot Description

LAMP1: Presents carbohydrate ligands to selectins. Also implicated in tumor cell metastasis. Belongs to the LAMP family.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 13q34

Cellular Component: lysosome; integral to plasma membrane; dendrite; multivesicular body; cell soma; membrane; cytoplasm; late endosome; plasma membrane; melanosome; endosome membrane; sarcolemma; external side of plasma membrane

Molecular Function: protein domain specific binding; protein binding; enzyme binding

Biological Process: positive regulation of natural killer cell degranulation; induction of apoptosis by granzyme; viral reproduction; protein stabilization; autophagic cell death; autophagy; positive regulation of natural killer cell mediated cytotoxicity