Rabbit anti-Human, Mouse Laminin alpha-5 Polyclonal Antibody | anti-N2402 antibody

Laminin alpha-5 (N2402) Polyclonal Antibody

Cat. Num. AAA3013610

• Reactivity: Human, Mouse
• Applications: Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: Laminin alpha-5; Polyclonal Antibody; Laminin alpha-5 (N2402) Polyclonal Antibody; LAMA5; Laminin alpha 5 chain; Laminin subunit alpha 5; Laminin subunit alpha-5; Laminin; alpha 5; anti-N2402 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Laminin alpha-5 (N2402) polyclonal antibody detects endogenous levels of Laminin alpha-5 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 3695

• Applicable Applications for anti-N2402 antibody: Immunohistochemistry (IHC)
• Application Notes: IHC: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 2371-2420 of Human Laminin alpha-5.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of Laminin alpha-5 (N2402) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.)

Related Product Information for anti-N2402 antibody

Laminins are essential and abundant structural non-collagenous glycoproteins localizing to basement membranes. Basement membranes (cell-associated extracellular matrices (ECMs)) are polymers of laminins with stabilizing type IV collagen networks, nidogen, and several proteoglycans. Basement membranes are found under epithelial layers, around the endothelium of blood vessels, and surrounding muscle, peripheral nerve, and fat cells. Formation of basement membranes influences cell proliferation, phenotype, migration, gene expression, and tissue architecture. Each laminin is a heterotrimer of alpha, beta, and gamma chain subunits that undergoes cell-secretion and incorporation into the ECM. Laminins can self-assemble, bind to other matrix macromolecules, and have unique and shared cell interactions mediated by Integrins, dystroglycan and cognate laminin receptors.

NCBI and Uniprot Product Information

• NCBI GI #: 21264602
• NCBI GeneID: 3911
• NCBI Accession #: NP_005551.3
• NCBI GenBank Nucleotide #: NP_005551.3
• UniProt Accession #: O15230
• Molecular Weight: ~ 400kDa
• NCBI Official Full Name: laminin subunit alpha-5
• NCBI Official Synonym Full Names: laminin subunit alpha 5
• NCBI Official Symbol: LAMA5
• NCBI Protein Information: laminin subunit alpha-5
• UniProt Protein Name: Laminin subunit alpha-5
• UniProt Gene Name: LAMA5
• UniProt Synonym Gene Names: KIAA0533; KIAA1907
• UniProt Entry Name: LAMA5_HUMAN

NCBI Description

This gene encodes one of the vertebrate laminin alpha chains. Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins are composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively) and they form a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. The protein encoded by this gene is the alpha-5 subunit of of laminin-10 (laminin-511), laminin-11 (laminin-521) and laminin-15 (laminin-523). [provided by RefSeq, Jun 2013]

Uniprot Description

LAMA5: Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.

Protein type: Secreted, signal peptide; Secreted; Extracellular matrix; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 20q13.2-q13.3

Cellular Component: extracellular matrix; extracellular space; laminin-5 complex; laminin-1 complex; laminin-10 complex; extracellular region; basement membrane; basal lamina; nucleus

Molecular Function: integrin binding; structural molecule activity

Biological Process: integrin-mediated signaling pathway; regulation of cell adhesion; focal adhesion formation; endothelial cell differentiation; extracellular matrix organization and biogenesis; muscle development; cell migration; regulation of embryonic development; cell recognition; cytoskeleton organization and biogenesis; regulation of cell migration; regulation of cell proliferation; odontogenesis of dentine-containing teeth; cell proliferation; extracellular matrix disassembly; embryonic development; morphogenesis of embryonic epithelium; hair follicle development; ureteric bud branching; cilium biogenesis; angiogenesis; cell differentiation; neural crest cell migration; morphogenesis of a polarized epithelium; lung development