Rabbit anti-Human, Mouse LAG3 Polyclonal Antibody | anti-LAG3 antibody

LAG3 Antibody

Cat. Num. AAA154461

• Gene Names: LAG3; CD223
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot
• Purity: Affinity chromatography purified via peptide column.
• Synonyms: LAG3; Polyclonal Antibody; LAG3 Antibody; lymphocyte activating 3; LAG-3; CD223; anti-LAG3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: At least two antibodies are known to exist; this antibody will only detect the largest isoform.
• Purity/Purification: Affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 525

• Applicable Applications for anti-LAG3 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: Western blot at 1 - 2 mug/mL.
• Conjugate: Unconjugated
• Immunogen: LAG3 antibody was raised against a peptide corresponding to 18 amino acids near the amino terminus of human LAG3.; The immunogen is located within amino acids 110 - 160 of LAG3.
• Buffer: LAG3 Antibody is supplied in PBS containing 0.02% sodium azide.
• Species: Human
• Preparation and Storage: Store at 4 degree C for three months and -20 degree C, stable for up to one year.; As with all antibodies care should be taken to avoid repeated freeze thaw cycles; Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of LAG3 in mouse liver tissue lysate with LAG3 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.)

Immunohistochemistry (IHC)

(Immunohistochemistry of LAG3 in human tonsil tissue with LAG3 antibody at 5 μg/ml.)

Immunofluorescence (IF)

(Immunofluorescence of LAG3 in human tonsil tissue with LAG3 antibody at 20 μg/ml.Red: LAG3 Antibody (8655)Blue: DAPI staining)

Related Product Information for anti-LAG3 antibody

The lymphocyte activation gene-3 (LAG3) is a member of the immunoglobulin superfamily and binds MHC class II with high affinity (1), negatively regulating T-cell function and homeostasis (2). It is expressed in B, T, and NK cells, monocytes, and dendritic cells (3), and acts to regulate T cell expansion (4). LAG3 is also an important immune checkpoint protein, with anti-LAG3 antibodies activating T effector cells and affecting regulatory T cell functions. Furthermore LAG3 appears to act in a synergistic fashion with PD-1/PD-L1, suggesting that a dual antibody approach may prove useful in cancer immunotherapy (5).

Product Categories/Family for anti-LAG3 antibody

Cell Cycle; Cancer; Immunology; Immune Checkpoint

References

1. Huard B, Tournier M, Hercend T, et al. Lymphocyte-activation gene 3/major histocompatibility complex class II interaction modulates the antigenic response of CD4+ T lymphocytes. Eur J Immunol 1994; 24:3216-21. 2. Triebel F. LAG-3: a regulator of T-cell and DC responses and its use in therapeutic vaccination. Trends Immunol 2003; 24:619-22. 3. Workman CJ, Wang Y, El Kasmi KC, et al. LAG-3 regulates plasmacytoid dendritic cell homeostasis. J Immunol. 2009; 182:1885-91. 4. Workman CJ, Vignali DA. The CD4-related molecule, LAG-3 (CD223), regulates the expansion of activated T cells. Eur J Immunol 2003; 33:970-9.

NCBI and Uniprot Product Information

• NCBI GI #: 167614500
• NCBI GeneID: 3902
• NCBI Accession #: NP_002277
• NCBI GenBank Nucleotide #: NM_002286.5
• UniProt Accession #: P18627; Q7Z643; A8K7T9
• Molecular Weight: Predicted: 55 kDa; Observed: 54 kDa
• NCBI Official Full Name: lymphocyte activation gene 3 protein
• NCBI Official Synonym Full Names: lymphocyte activating 3
• NCBI Official Symbol: LAG3
• NCBI Official Synonym Symbols: CD223
• NCBI Protein Information: lymphocyte activation gene 3 protein
• UniProt Protein Name: Lymphocyte activation gene 3 protein
• Protein Family: Lymphocyte activation gene 3 protein
• UniProt Gene Name: LAG3
• UniProt Synonym Gene Names: FDC; LAG-3

NCBI Description

Lymphocyte-activation protein 3 belongs to Ig superfamily and contains 4 extracellular Ig-like domains. The LAG3 gene contains 8 exons. The sequence data, exon/intron organization, and chromosomal localization all indicate a close relationship of LAG3 to CD4. [provided by RefSeq, Jul 2008]

Uniprot Description

LAG3: a cell surface receptor involved in lymphocyte activation and neural function. LAG3 expressed on neurons is a receptor that binds extracellular Alpha-synuclein fibrils (PFF) with high affinity, leading to its endocytosis. LAG3 facilitates the neuron-to-neuron spread of PFF and its associated neural toxicity. Binds to HLA class-II antigens. Two isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 12p13.31

Cellular Component: plasma membrane

Molecular Function: antigen binding; MHC class II protein binding

Biological Process: antigen processing and presentation of exogenous peptide antigen via MHC class II