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Western Blot (WB)
(Western Blot: Sample: Lane1: Rat Serum; Lane2: Rat Brain Tissue; Lane3: Rat Liver Tissue; Lane4: Rat Testis Tissue.)
Rabbit anti-Rat Lactate Dehydrogenase C Polyclonal Antibody | anti-LDHC antibody
Polyclonal Antibody to Lactate Dehydrogenase C (LDHC)
Gene Names
Ldhc; Ldh3
Reactivity
Rat
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA
Purity
Affinity Chromatography
Synonyms
Lactate Dehydrogenase C; Polyclonal Antibody; Polyclonal Antibody to Lactate Dehydrogenase C (LDHC); anti-LDHC antibody
Host
Rabbit
Reactivity
Rat
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against LDHC. It has beenselected for its ability to recognize LDHC in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Liquid
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- MSTVKEQLIQ NLAPDEKQSR CKITVVGVGN VGMACAISIL LKGLADELAL VDADENKLKG EALDLLHGSL FLSTPKIVFG KDYSVSANSK LVIITAGARM VSGESRLALL QRNVTSMKAI VPGVIQNSPD CKIMIVTNPV DILTYVVWKI SGLPVSSVIG SGCNLDSARF RYLIGEKLGV NPSSCHGWVL GEHGDSSVPI WSGVNIAGVT LKSLNPAIGS DSDKEQWKTV HKQVVDGGYE VLNLKGYTSW AIALSVTDIA ASILKNLKRV HAVTTLVKGL YGIKEEIFLS IPCVLGQSGI TDLVKVNMNT EEEALFKKSC
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- MSTVKEQLIQ NLAPDEKQSR CKITVVGVGN VGMACAISIL LKGLADELAL VDADENKLKG EALDLLHGSL FLSTPKIVFG KDYSVSANSK LVIITAGARM VSGESRLALL QRNVTSMKAI VPGVIQNSPD CKIMIVTNPV DILTYVVWKI SGLPVSSVIG SGCNLDSARF RYLIGEKLGV NPSSCHGWVL GEHGDSSVPI WSGVNIAGVT LKSLNPAIGS DSDKEQWKTV HKQVVDGGYE VLNLKGYTSW AIALSVTDIA ASILKNLKRV HAVTTLVKGL YGIKEEIFLS IPCVLGQSGI TDLVKVNMNT EEEALFKKSC
Sequence Length
232
Applicable Applications for anti-LDHC antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes
Western blotting: 0.5-2ug/ml
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.
Fragment
LDHC (Met1~Leu332)
Organism Species
Rattus norvegicus (Rat)
Cross Reactivity
Rat
Conjugate
No Conjugate
Immunogen
Recombinant LDHC (Met1~Leu332) expressed in E Coli.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2073978
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample: Lane1: Rat Serum; Lane2: Rat Brain Tissue; Lane3: Rat Liver Tissue; Lane4: Rat Testis Tissue.)
Western Blot (WB)
(Western Blot: Sample: Lane1: Rat Serum; Lane2: Rat Brain Tissue; Lane3: Rat Liver Tissue; Lane4: Rat Testis Tissue.)
Western Blot (WB)
(Western Blot: Sample: Recombinant LDHC, Rat.)
Western Blot (WB)
(Western Blot: Sample: Recombinant LDHC, Rat.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Rat Testis Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Rat Testis Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Rat Testis Tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Rat Testis Tissue))
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
35,687 Da
NCBI Official Full Name
L-lactate dehydrogenase C chain
NCBI Official Synonym Full Names
lactate dehydrogenase C
NCBI Official Symbol
Ldhc
NCBI Official Synonym Symbols
Ldh3
NCBI Protein Information
L-lactate dehydrogenase C chain
UniProt Protein Name
L-lactate dehydrogenase C chain
Protein Family
UniProt Gene Name
Ldhc
UniProt Synonym Gene Names
Ldh-3; Ldh3; LDH-C
NCBI Description
member of the lactate dehydrogenase family, which catalyzes the conversion of lactate to pyruvate [RGD, Feb 2006]
Uniprot Description
LDH-C: Possible role in sperm motility. Belongs to the LDH/MDH superfamily. LDH family.
Protein type: Amino Acid Metabolism - cysteine and methionine; Cancer Testis Antigen (CTA); Carbohydrate Metabolism - glycolysis and gluconeogenesis; Carbohydrate Metabolism - propanoate; Carbohydrate Metabolism - pyruvate; EC 1.1.1.27; Oxidoreductase
Chromosomal Location of Human Ortholog: 1q22
Cellular Component: cilium; cytoplasm; nucleus
Molecular Function: L-lactate dehydrogenase activity; oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
Biological Process: ATP biosynthetic process; carbohydrate metabolic process; carboxylic acid metabolic process; lactate biosynthetic process from pyruvate; lactate oxidation; pyruvate metabolic process; sperm motility
Similar Products
Product Notes
The LDHC ldhc (Catalog #AAA2026673) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Lactate Dehydrogenase C (LDHC) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Lactate Dehydrogenase C can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 0.5-2ug/ml Immunocytochemistry in formalin fixed cells: 5-20ug/ml Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml Immunohistochemistry in paraffin section: 5-20ug/ml Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the LDHC ldhc for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- MSTVKEQLIQ NLAPDEKQSR CKITVVGVGN VGMACAISIL LKGLADELAL VDADENKLKG EALDLLHGSL FLSTPKIVFG KDYSVSANSK LVIITAGARM VSGESRLALL QRNVTSMKAI VPGVIQNSPD CKIMIVTNPV DILTYVVWKI SGLPVSSVIG SGCNLDSARF RYLIGEKLGV NPSSCHGWVL GEHGDSSVPI WSGVNIAGVT LKSLNPAIGS DSDKEQWKTV HKQVVDGGYE VLNLKGYTSW AIALSVTDIA ASILKNLKRV HAVTTLVKGL YGIKEEIFLS IPCVLGQSGI TDLVKVNMNT EEEALFKKSC. It is sometimes possible for the material contained within the vial of "Lactate Dehydrogenase C, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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