Western Blot (WB)
(Western blot analysis of KMO Antibody (Center) in CEM cell line lysates (35ug/lane). KMO (arrow) was detected using the purified Pab.)
Rabbit anti-Human Kynurenine 3-monooxygenase Polyclonal Antibody | anti-KMO antibody
Kynurenine 3-monooxygenase (KMO, dJ317G22.1, Kynurenine 3-hydroxylase)
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Dilution: ELISA: 1:1,000
Western Blot: 1:100-1:500
Western Blot (WB)
(Western blot analysis of KMO Antibody (Center) in CEM cell line lysates (35ug/lane). KMO (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of KMO Antibody (Center) in CEM cell line lysates (35ug/lane). KMO (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with KMO Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with KMO Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(KMO Antibody (Center) flow cytometry analysis of CEM cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(KMO Antibody (Center) flow cytometry analysis of CEM cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
NCBI and Uniprot Product Information
NCBI Description
Kynurenine 3-monooxygenase (KMO; EC 1.14.13.9) is an NADPH-dependent flavin monooxygenase that catalyzes the hydroxylation of the L-tryptophan metabolite L-kynurenine to form L-3-hydroxykynurenine.[supplied by OMIM]
Uniprot Description
KMO: Catalyzes the hydroxylation of L-kynurenine (L-Kyn) to form 3-hydroxy-L-kynurenine (L-3OHKyn). Required for synthesis of quinolinic acid, a neurotoxic NMDA receptor antagonist and potential endogenous inhibitor of NMDA receptor signaling in axonal targeting, synaptogenesis and apoptosis during brain development. Quinolinic acid may also affect NMDA receptor signaling in pancreatic beta cells, osteoblasts, myocardial cells, and the gastrointestinal tract. Belongs to the aromatic-ring hydroxylase family. KMO subfamily. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Mitochondrial; Amino Acid Metabolism - tryptophan; Membrane protein, multi-pass; Membrane protein, integral; Oxidoreductase; EC 1.14.13.9
Chromosomal Location of Human Ortholog: 1q42-q44
Cellular Component: mitochondrial outer membrane; mitochondrion; mitochondrial inner membrane; integral to membrane; cytosol
Molecular Function: FAD binding; NAD(P)H oxidase activity; kynurenine 3-monooxygenase activity
Biological Process: anthranilate metabolic process; tryptophan catabolic process to kynurenine; NAD metabolic process; quinolinate biosynthetic process; tryptophan catabolic process; response to salt stress
Research Articles on KMO
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Product Notes
The KMO kmo (Catalog #AAA628299) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Kynurenine 3-monooxygenase (KMO, dJ317G22.1, Kynurenine 3-hydroxylase) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Kynurenine 3-monooxygenase can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in ELISA and Western Blot. Dilution: ELISA: 1:1,000 Western Blot: 1:100-1:500. Researchers should empirically determine the suitability of the KMO kmo for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Kynurenine 3-monooxygenase, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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